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首页> 外文期刊>Infection and immunity >Characterization of a Mycobacterium leprae antigen related to the secreted Mycobacterium tuberculosis protein MPT32.
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Characterization of a Mycobacterium leprae antigen related to the secreted Mycobacterium tuberculosis protein MPT32.

机译：与分泌的结核分枝杆菌蛋白MPT32有关的麻风分枝杆菌抗原的表征。

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Secreted proteins may serve as major targets in the immune response to mycobacteria. To identify potentially secreted Mycobacterium leprae antigens, antisera specific for culture filtrate proteins of Mycobacterium tuberculosis were used to screen a panel of recombinant antigens selected previously by leprosy patient sera. Four potentially secreted antigens were identified by this approach, and one was recognized by antibodies specific for MPT32, a secreted M. tuberculosis protein. The DNA coding for the M. leprae antigen, which we have designated 43L, was isolated and characterized and found to encode a 25.5-kDa protein that is preceded by a consensus signal peptide of 39 amino acids. The N-terminal amino acid sequence of 43L shows 50% homology with the 20 known N-terminal amino acids of MPT32, and 47% homology was found with the N terminus of a 45/47-kDa antigen complex identified in Mycobacterium bovis BCG. These findings indicate that 43L represents an antigen related to MPT32 and the M. bovis BCG 45/47-kDa complex and that 43L is likely to be a protein secreted by M. leprae. Purified recombinant 43L protein is recognized by antibodies and T cells from healthy contacts and leprosy patients, illustrating that secreted proteins are of importance in the immune response to M. leprae.
机译：分泌的蛋白质可以作为对分枝杆菌的免疫反应的主要靶标。为了鉴定潜在分泌的麻风分枝杆菌抗原，对结核分枝杆菌的培养滤液蛋白具有特异性的抗血清用于筛选麻风病人血清先前选择的一组重组抗原。通过这种方法鉴定了四种可能分泌的抗原，一种被被分泌的结核分枝杆菌蛋白MPT32特异性的抗体识别。分离并鉴定了编码麻风分枝杆菌抗原的DNA（我们将其命名为43L），发现其编码为25.5 kDa蛋白，该蛋白之前带有39个氨基酸的共有信号肽。 43L的N末端氨基酸序列与MPT32的20个已知N末端氨基酸具有50％的同源性，在牛分枝杆菌BCG中鉴定出的45 / 47-kDa抗原复合物的N末端具有47％的同源性。这些发现表明43L代表与MPT32和牛分枝杆菌BCG 45 / 47-kDa复合物有关的抗原，并且43L很可能是麻风分枝杆菌分泌的蛋白质。纯化的重组43L蛋白被健康接触者和麻风病患者的抗体和T细胞识别，这说明分泌的蛋白在对麻风分枝杆菌的免疫反应中很重要。

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