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首页> 外文期刊>Infection and immunity >The pef fimbrial operon of Salmonella typhimurium mediates adhesion to murine small intestine and is necessary for fluid accumulation in the infant mouse.
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The pef fimbrial operon of Salmonella typhimurium mediates adhesion to murine small intestine and is necessary for fluid accumulation in the infant mouse.

机译：鼠伤寒沙门氏菌的pef纤维操纵子介导与鼠小肠的粘附，是婴儿小鼠体内积液的必要条件。

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摘要

We investigated the role of the pef operon, containing the genes for plasmid-encoded (PE) fimbriae of Salmonella typhimurium, in adhesion to the murine small intestine. In an organ culture model, a mutant of S. typhimurium carrying a tetracycline resistance cassette inserted in pefC was found to be associated in lower numbers with murine small intestine than the wild-type. Similarly, heterologous expression of PE fimbriae in Escherichia coli increased the bacterial numbers recovered from the intestine in the organ culture model. Adhesion to villous intestine mediated by PE fimbriae was further demonstrated by binding of an E. coli strain expressing PE fimbriae to thin sections of mouse small intestine. The contribution of pef-mediated adhesion on fluid accumulation was investigated in infant mice. Intragastric injection of S. typhimurium 14028 and SR-11 caused fluid accumulation in infant mice. In contrast, pefC mutants of S. typhimurium 14028 and SR-11 were negative in the infant mouse assay. Introduction of a plasmid containing pefBACD and orf5, the first five genes of the pef operon, into the pefC mutant complemented for fluid accumulation in the infant mouse assay. However, heterologous expression of PE fimbriae in E. coli did not result in fluid accumulation in the infant mouse, suggesting that factors other than fimbriae are involved in causing fluid accumulation.
机译：我们调查了含有鼠伤寒沙门氏菌质粒编码（PE）菌毛基因的pef操纵子在与鼠小肠粘连中的作用。在器官培养模型中，鼠伤寒沙门氏菌的突变体携带了插入pefC的四环素抗性盒，与野生型鼠小肠的相关性较低。同样，PE菌毛在大肠杆菌中的异源表达增加了器官培养模型中从肠道中回收的细菌数量。通过表达PE菌毛的大肠杆菌菌株与小鼠小肠的薄片结合，进一步证明了由PE菌毛介导的对绒毛肠的粘附。在婴儿小鼠中研究了pef介导的粘附对体液积聚的贡献。胃内注射鼠伤寒沙门氏菌14028和SR-11导致婴儿小鼠体内积液。相反，鼠伤寒沙门氏菌14028和SR-11的pefC突变体在婴儿小鼠试验中为阴性。将包含pefBACD和orf5（pef操纵子的前五个基因）的质粒引入pefC突变体，以补充婴儿小鼠实验中的体液积聚。但是，PE菌毛在大肠杆菌中的异源表达并未导致婴儿小鼠体内积液，这表明除菌毛以外的其他因素也引起了积液。

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