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首页> 外文期刊>Infection and immunity >Antigenic heterogeneity and molecular analysis of CopB of Moraxella (Branhamella) catarrhalis.
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Antigenic heterogeneity and molecular analysis of CopB of Moraxella (Branhamella) catarrhalis.

机译：卡他莫拉氏菌（Branhamella）卡他氏菌CopB的抗原异质性和分子分析。

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摘要

Outer membrane protein (OMP) CopB, an iron-repressible 81-kDa major OMP of Moraxella (Branhamella) catarrhalis has been a major focus of investigation. To assess CopB as a potential vaccine antigen, we elucidated the degree of antigenic and sequence heterogeneity in this protein among strains of M. catarrhalis. Two monoclonal antibodies, 1F5 and 2.9F, which bind to surface-exposed epitopes on CopB recognized 60 and 70% of the strains, respectively. The degree of sequence heterogeneity in CopB was assessed by cloning and sequencing the CopB gene from two different strains of M. catarrhalis and comparing with the published sequence. There was 92 to 96% homology between the sequences at the nucleotide level and 90 to 95% homology at the amino acid level. The variability in the protein sequence is confined mainly to three moderately variable regions. Restriction fragment length polymorphism (RFLP) analysis of the CopB genes obtained from 20 diverse strains by PCR was performed. Ninety percent of the potential restriction sites in the constant regions and 47% of the potential restriction sites in the variable regions were present in the 20 strains, indicating that the pattern of variable and constant areas in the CopB gene is a general pattern among strains of M. catarrhalis. We conclude that the CopB gene is largely conserved among strains of M. catarrhalis and contains discrete regions which show moderate heterogeneity among strains.
机译：外膜蛋白（OMP）CopB是一种铁可抑制的卡他莫拉氏菌（Branhamella）的81 kDa主要OMP，一直是研究的重点。为了评估CopB作为一种潜在的疫苗抗原，我们阐明了该基因在卡他莫拉氏菌菌株之间的抗原性和序列异质性程度。与CopB上表面暴露的表位结合的两种单克隆抗体1F5和2.9F分别识别了60％和70％的菌株。 CopB中序列异质性的程度通过克隆和测序两种粘膜炎莫拉氏菌菌株的CopB基因并与已发表的序列进行比较来评估。在核苷酸水平上的序列之间具有92％至96％的同源性，而在氨基酸水平上的序列之间具有90％至95％的同源性。蛋白质序列的可变性主要限于三个中等可变区。通过PCR对20种不同菌株获得的CopB基因进行了限制性片段长度多态性（RFLP）分析。在20个菌株中存在恒定区的90％的潜在限制性酶切位点和在可变区的47％的潜在限制性酶切位点，表明CopB基因的可变区和恒定区的模式是普通菌株中的一般模式。卡他氏菌。我们得出的结论是，CopB基因在粘膜炎莫拉氏菌菌株之间大部分是保守的，并且包含在菌株之间显示中等异质性的离散区域。

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