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首页> 外文期刊>Infection and immunity >The Lipopolysaccharide of Bordetella bronchiseptica Acts as a Protective Shield against Antimicrobial Peptides
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The Lipopolysaccharide of Bordetella bronchiseptica Acts as a Protective Shield against Antimicrobial Peptides

机译：支气管杆菌博德特氏菌的脂多糖起抗菌肽的保护作用

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Resistance profiles of the two Bordetella speciesB. bronchiseptica and B. pertussis against various antimicrobial peptides were determined in liquid survival and agar diffusion assays. B. bronchiseptica exhibited significantly higher resistance against all tested peptides thanB. pertussis. The most powerful agents acting on B. bronchiseptica were, in the order of their killing efficiencies, cecropin P > cecropin B > magainin-II-amide > protamine > melittin. Interestingly, for B. bronchiseptica, the resistance level was significantly affected by phase variation, as a bvgS deletion derivative showed an increased sensitivity to these peptides. Tn5-induced protamine-sensitive B. bronchiseptica mutants, which were found to be very susceptible to most of the cationic peptides, were isolated. In two of these mutants, the genetic loci inactivated by transposon insertion were identified as containing genes highly homologous to the wlbA and wlbL genes ofB. pertussis that are involved in the biosynthesis of lipopolysaccharide (LPS). In agreement with this finding, the two peptide-sensitive mutants revealed structural changes in the LPS, resulting in the loss of the O-specific side chains and the prevalence of the LPS core structure. This demonstrates that LPS plays a major role in the resistance of B. bronchiseptica against the action of antimicrobial peptides and suggests that B. pertussis is much more susceptible to these peptides due to the lack of the highly charged O-specific sugar side chains.
机译：两种 Bordetella 菌种 B的抗性概况。支气管败血病和 B。在液体存活和琼脂扩散试验中确定了百日咳对各种抗菌肽的抵抗力。 B。支气管败血病对所有测试肽的抵抗力均显着高于 B。百日咳。对 B起作用的最强大的特工。支气管败血病按照其杀灭效率的顺序依次为：头孢克酮P>头孢克灵B>洋牛苷II-酰胺>鱼精蛋白>蜂毒肽。有趣的是，对于 B。支气管败血病中，抗性水平受相变的影响很大，因为 bvgS 缺失衍生物对这些肽的敏感性增加。 Tn 5 诱导的鱼精蛋白敏感性 B。分离出对大多数阳离子肽都非常敏感的支气管败血病突变体。在其中两个突变体中，经转座子插入而失活的基因位点被鉴定为包含与 B的 wlbA 和 wlbL 基因高度同源的基因。百日咳与脂多糖（LPS）的生物合成有关。与这一发现相一致的是，这两个对肽敏感的突变体揭示了LPS的结构变化，导致O特异侧链的丢失和LPS核心结构的普遍性。这表明LPS在 B的抗性中起主要作用。支气管败血病对抗抗菌肽的作用，提示 B。百日咳由于缺乏高电荷的O-特异性糖侧链而对这些肽敏感。

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