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Gene Discovery through Expressed Sequence Tag Sequencing in Trypanosoma cruzi

机译：克鲁氏锥虫通过表达序列标签测序的基因发现。

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Analysis of expressed sequence tags (ESTs) constitutes a useful approach for gene identification that, in the case of human pathogens, might result in the identification of new targets for chemotherapy and vaccine development. As part of the Trypanosoma cruzigenome project, we have partially sequenced the 5′ ends of 1,949 clones to generate ESTs. The clones were randomly selected from a normalized CL Brener epimastigote cDNA library. A total of 14.6% of the clones were homologous to previously identified T. cruzi genes, while 18.4% had significant matches to genes from other organisms in the database. A total of 67% of the ESTs had no matches in the database, and thus, some of them might be T. cruzi-specific genes. Functional groups of those sequences with matches in the database were constructed according to their putative biological functions. The two largest categories were protein synthesis (23.3%) and cell surface molecules (10.8%). The information reported in this paper should be useful for researchers in the field to analyze genes and proteins of their own interest.
机译：表达序列标签（EST）的分析构成了一种有用的基因鉴定方法，在人类病原体的情况下，可能导致鉴定化学疗法和疫苗开发的新靶标。作为克氏锥虫基因组计划的一部分，我们对1,949个克隆的5'端进行了部分测序，以产​​生EST。从归一化的CL Brener附睾基因cDNA文库中随机选择克隆。总共14.6％的克隆与先前鉴定的 T同源。 Cruzi 基因，而18.4％的基因与数据库中其他生物的基因具有显着匹配。数据库中总共有67％的EST没有匹配项，因此其中一些可能是 T。 Cruzi特异基因。这些数据库中具有匹配项的序列的功能组是根据其假定的生物学功能构建的。最大的两个类别是蛋白质合成（23.3％）和细胞表面分子（10.8％）。本文报道的信息对于本领域的研究人员分析自己感兴趣的基因和蛋白质应该是有用的。

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