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首页> 外文期刊>Infection and immunity >Vaccination with Cathepsin L Proteinases and with Leucine Aminopeptidase Induces High Levels of Protection against Fascioliasis in Sheep
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Vaccination with Cathepsin L Proteinases and with Leucine Aminopeptidase Induces High Levels of Protection against Fascioliasis in Sheep

机译：组织蛋白酶L蛋白酶和亮氨酸氨基肽酶的疫苗接种可诱导绵羊抵抗筋膜病的高水平保护

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The potential of different parasite proteinases for use as vaccine candidates against fascioliasis in sheep was studied by vaccinating animals with the cathepsin L proteinases CL1 and CL2 and with leucine aminopeptidase (LAP) purified from adult flukes. In the first trial, sheep were immunized with CL1 or CL2 and the mean protection levels obtained were 33 and 34%, respectively. Furthermore, a significant reduction in egg output was observed in sheep vaccinated either with CL1 (71%) or with CL2 (81%). The second trial was performed to determine the protective potential of the two cathepsin L proteinases assayed together, as well as in combination with LAP, and of LAP alone. The combination of CL1 and CL2 induced higher levels of protection (60%) than those produced when these enzymes were administered separately. Those sheep that received the cocktail vaccine including CL1, CL2, and LAP were significantly protected (78%) against metacercarial challenge, but vaccination with LAP alone elicited the highest level of protection (89%). All vaccine preparations induced high immunoglobulin G titers which were boosted after the challenge infection, but no correlations between antibody titers and worm burdens were found. However, the sera of those animals vaccinated with LAP contained LAP-neutralizing antibodies. Reduced liver damage, as assessed by the level of the liver enzyme gamma-glutamyl transferase, was observed in the groups vaccinated with CL1, CL2, and LAP or with LAP alone.
机译：通过用组织蛋白酶L蛋白酶CL1和CL2以及从成年吸虫中纯化的亮氨酸氨肽酶（LAP）接种动物，研究了不同的寄生虫蛋白酶在羊中作为抵抗筋膜炎的候选疫苗的潜力。在第一个试验中，用CL1或CL2免疫绵羊，获得的平均保护水平分别为33％和34％。此外，在接种了CL1（71％）或CL2（81％）的绵羊中，观察到产蛋量显着下降。进行了第二次试验，以确定一起测定的两种组织蛋白酶L蛋白酶以及与LAP联合测定以及单独使用LAP的保护潜力。与单独施用这些酶时相比，CL1和CL2的组合诱导的保护水平更高（60％）。那些接受了包括CL1，CL2和LAP在内的混合疫苗的绵羊得到了显着的保护（78％）以防meta虫攻击，但是仅用LAP进行疫苗接种可以带来最高的保护水平（89％）。所有疫苗制剂均诱导高免疫球蛋白G滴度，在攻击感染后可提高该滴度，但未发现抗体滴度与蠕虫负担之间的相关性。但是，接种了LAP的那些动物的血清中含有LAP中和抗体。在用CL1，CL2和LAP或单独使用LAP接种的组中，通过肝酶γ-谷氨酰转移酶的水平评估，可以减少肝脏损害。

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