TetR Is a Positive Regulator of the Tetanus Toxin Gene in Clostridium tetani and Is Homologous to BotR

Jean-Christophe Marvaud; Ulrich Eisel; Thomas Binz; Heiner Niemann; Michel R. Popoff

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TetR Is a Positive Regulator of the Tetanus Toxin Gene in Clostridium tetani and Is Homologous to BotR

机译：TetR是破伤风梭菌破伤风毒素基因的正向调节剂，与BotR同源

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The TetR gene immediately upstream from the tetanus toxin (TeTx) gene was characterized. It encodes a 21,562-Da protein which is related (50 to 65% identity) to the equivalent genes (botR) inClostridium botulinum. TetR has the feature of a DNA binding protein with a basic pI (9.53). It contains a helix-turn-helix motif and shows 29% identity with other putative regulatory genes inClostridium, i.e., uviA from C. perfringens and txeR from C. difficile. We report for the first time the transformation of C. tetani by electroporation, which permitted us to investigate the function of tetR. Overexpression of tetR inC. tetani induced an increase in TeTx production and in the level of the corresponding mRNA. This indicates that TetR is a transcriptional activator of the TeTx gene. Overexpression ofbotR/A (60% identity with TetR at the amino acid level) inC. tetani induced an increase in TeTx production comparable to that for overexpression of tetR. However,botR/C (50% identity with TetR at the amino acid level) was less efficient. This supports that TetR positively regulates the TeTx gene in C. tetani and that a conserved mechanism of regulation of the neurotoxin genes is involved in C. tetaniand C. botulinum.

机译：破伤风毒素（TeTx）基因上游的TetR基因的特征。它编码一个21,562-Da的蛋白质，与肉毒梭状芽胞杆菌的等效基因（ botR ）相关（50％到65％）。 TetR具有带有碱性pI（9.53）的DNA结合蛋白的特征。它包含一个螺旋-转-螺旋基序，并与 Clostridium 中其他推定的调节基因，即来自 C的 uviA 显示29％的同一性。来自 C的perfringens 和 txeR 。困难。我们首次报告了 C的转化。 tetani 通过电穿孔，这使我们能够研究 tetR 的功能。 tetR 在 C中的过表达。 tetani 诱导了TeTx产生和相应mRNA水平的增加。这表明TetR是TeTx基因的转录激活因子。 C中 botR / A 的过表达（在氨基酸水平上与TetR具有60％的同一性）。 tetani 诱导的TeTx产量增加与 tetR 的过表达相当。但是， botR / C （在氨基酸水平上与TetR的同一性为50％）效率较低。这支持TetR积极调节 C中的TeTx基因。破伤风，并且 C参与了神经毒素基因的保守调节机制。 tetani 和 C。肉毒杆菌素。 展开▼

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《Infection and immunity》 |1998年第12期|共5页

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Jean-Christophe Marvaud; Ulrich Eisel; Thomas Binz; Heiner Niemann; Michel R. Popoff;
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中图分类医学免疫学;

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1. BotR/A and TetR are alternative RNA polymerase sigma factors controlling the expression of the neurotoxin and associated protein genes in Clostridium botulinum type A and Clostridium tetani. [J] . Raffestin S, Dupuy B, Marvaud JC, Molecular Microbiology . 2005,第1期

机译：BotR / A和TetR是另一种RNA聚合酶sigma因子，可控制A型肉毒梭菌和破伤风梭菌中神经毒素和相关蛋白基因的表达。

2. botR/A is a positive regulator of botulinum neurotoxin and associated non-toxin protein genes in Clostridium botulinum A. [J] . Marvaud JC, Gibert M, Inoue K, Molecular Microbiology . 1998,第4期

机译：botR / A是肉毒梭菌A中肉毒杆菌神经毒素和相关非毒素蛋白基因的正向调节剂。

3. Tetanus Toxin Synthesis is Under the Control of A Complex Network of Regulatory Genes in Clostridium tetani [J] . Diana Chapeton-Montes, Lucile Plourde, Cecile Deneve, Toxins . 2020,第5期

机译：破伤风毒素合成是在Tetani梭菌中的调控基因复杂网络的控制下

4. A characterization of epileptogenesis presented in hippocampal neural activity in a rat tetanus toxin model [C] . Sang-Eon Park, Mark J. Connolly, Robert E. Gross Annual International Conference of the IEEE Engineering in Medicine and Biology Society . 2019

机译：在大鼠破伤风毒素模型中海马神经活动中癫痫发生的特征

5. Development of peptide inhibitors targeting Clostridium difficile toxins A/B and characterizing the regulatory role of a putative negative regulator TcdC in Clostridium difficile toxin gene expression. [D] . Abdeen, Sanofar J. 2013

机译：靶向艰难梭菌毒素A / B的肽抑制剂的开发以及表征推定的负调节剂TcdC在艰难梭菌毒素基因表达中的调节作用。

6. TetR Is a Positive Regulator of the Tetanus Toxin Gene in Clostridium tetani and Is Homologous to BotR [O] . Jean-Christophe Marvaud, Ulrich Eisel, Thomas Binz, 1998

机译：TetR是破伤风梭菌破伤风毒素基因的正向调节剂与BotR同源

7. TetR Is a Positive Regulator of the Tetanus Toxin Gene in Clostridium tetani and Is Homologous to BotR [O] . Jean-Christophe Marvaud, Ulrich Eisel, Thomas Binz, 1998

机译：TetR是破伤风梭菌中破伤风毒素基因的正调节因子，与BotR同源

8. Genetic Engineering of Clostridium Difficile Toxin A Vaccine [R] . Muldrow, L. L., Johnson, J. 1991

机译：艰难梭菌毒素a疫苗的基因工程

1. TetR家族转录调控基因tfpA对链霉菌合成A21978C的影响 [J] . 逄丽 ,魏维 ,靳旭 . 中国抗生素杂志 . 2015,第005期

2. 载体中tetR和ampR抗性检验基因及蓝白筛选原理 [J] . 孙伟男 ,张玉 . 畜牧与饲料科学 . 2013,第004期

3. 吸水链霉菌TetR基因的克隆与表达 [J] . 王恒安 ,秦磊 ,庞志轩 . 吉林农业大学学报 . 2004,第003期

4. 前B细胞白血病同源盒基因3、磷酸酶和张力蛋白同源等位基因在宫颈癌中的表达及与临床病理特征和预后的关系 [J] . 姜梦婕 ,江利 ,韩克 . 中国医师进修杂志 . 2021,第004期

5. 远中缺失同源盒基因5和肌节同源盒基因1在双膦酸盐药物性颌骨坏死大鼠模型中的变化 [J] . 张文怡 ,轩宾 ,郭宇轩 . 中华口腔医学杂志 . 2018,第007期

6. 破伤风毒素C片段基因的克隆及其在大肠杆菌中的高效表达 [C] . 刘婷 ,焦新安 ,潘志明 . 中国畜牧兽医学会家畜传染病学分会第六届全国会员代表大会暨第11次学术研讨会 . 2005

7. 新型AMPA受体正向变构调节剂PF-4778574抗抑郁作用分子机制 [A] . 李蒙蒙 . 2018

1. 具有正向调控植株侧生器官大小的INP1基因及其同源基因 [P] . 中国专利： CN111349633A . 2020-06-30

2. 基于TetR蛋白空间位阻和基因剪切技术的四环素类药物检测方法 [P] . 中国专利： CN113957067A . 2022-01-21

3. Fusion protein Fusion protein The soluble composition of the host cell material Process to generate a neutralizing anti-toxin directed against Clostridium botulinum toxin type A antibody to purify a recombinant fusion protein derived from a Clostridium botulinum toxin type A process to generate an antitoxin neutralizing directed against clostridium difficile toxin type b antibody treatment process process to generate a neutralizing antitoxin directed against clostridium difficile type a toxin composition process to vaccinate an individual to produce neutralizing antitoxin directed against clostridium difficile toxin processing the detection of clostridium antigens difficile in an ample process of purification of toxins from clostridium difficile from a culture and process of generation of a solid dosage form of a bird antitoxin directed against a protein of clostridial toxin [P] . 外国专利： BR9509903A . 1997-11-25

机译：融合蛋白融合蛋白宿主细胞材料的可溶性组成物产生针对A型肉毒梭菌毒素的中和抗毒素的过程纯化源自A型肉毒杆菌毒素的重组融合蛋白的抗体产生针对人肉毒杆菌的抗毒素的过程B型艰难梭菌毒素抗体处理工艺过程，产生针对A艰难梭菌毒素的中和抗毒素组合物，对个体进行疫苗接种，以生产针对艰难梭菌毒素的中和抗毒素，在充足的纯化纯化过程中检测艰难梭菌抗原来源于梭状芽胞杆菌毒素蛋白的鸟抗毒素固体剂型的培养物和固体剂型产生过程中的艰难梭菌毒素

4. Detoxified immunogenic derivative of clostridium perfringens b-toxin or an immunogenic fragment thereof, nucleotide sequence comprising a mutant DNA fragment, clostriduim perfringensm expression system based on a gram-positive bacterium, vaccine to combat clostridium perfrigens infection, process for the preparation of native b-toxin from clostridium perfrigens, and, process for preparing a vaccine to combat infection of clostridium perfrigens. [P] . 外国专利： BR9802361A . 2000-01-11

机译：产气荚膜梭菌b毒素的解毒免疫原性衍生物或其免疫原性片段，包含突变DNA片段的核苷酸序列，基于革兰氏阳性细菌的产气荚膜梭菌表达系统，抗付产气荚膜梭菌感染的疫苗，制备天然b-产高产梭状芽胞杆菌的毒素，以及制备疫苗来抵抗产高产梭状芽胞杆菌感染的方法。

5. BACTERIAL TOXIN NEUTRALIZER FOR CLOSTRIDIUM BOTULINUM OR TETANUS BACILLUS, AND INTOXICATION PREVENTING AGENT AND FOOD AND DRINK EACH USING THE SAME [P] . 外国专利： JP2005237267A . 2005-09-08

机译：肉毒杆菌或破伤风杆菌的细菌毒素中和剂，以及使用它们的抗氧化剂，食品和饮料

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TetR Is a Positive Regulator of the Tetanus Toxin Gene in Clostridium tetani and Is Homologous to BotR

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