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首页> 外文期刊>Infection and immunity >Nucleotide Sequences of Genes Coding for Fimbrial Proteins in a Cryptic Genospecies of Haemophilus spp. Isolated from Neonatal and Genital Tract Infections
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Nucleotide Sequences of Genes Coding for Fimbrial Proteins in a Cryptic Genospecies of Haemophilus spp. Isolated from Neonatal and Genital Tract Infections

机译：嗜血杆菌属隐种基因组中编码膜蛋白基因的核苷酸序列。分离自新生儿和生殖道感染

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Nineteen isolates belonging to a cryptic genospecies ofHaemophilus (referred to here as genital strains) isolated from genital tract infections (6 strains) and from neonatal infections (13 strains) were studied for fimbrial genes. Sixteen strains exhibit peritrichous fimbriae observed by electron microscopy. By PCR with primers corresponding to the extreme ends of the Haemophilus influenzae type b (Hib) hifA and hifDgenes and Southern blotting, a hifA-like gene (namedghfA) and a hifD-like gene (namedghfD) were identified in 6 of the 19 strains. Five of these six strains were from the genital tracts of adults, and one was from a neonate. For each gene, the nucleotide sequence was identical for the six strains. A hifE-like gene (named ghfE) was amplified from only one of the 19 genital strains ofHaemophilus, but the ghfE probe gave a signal in Southern hybridization with the five other strains positive forghfA and ghfD. Therefore, these strains may carry a ghfE-like gene. The Hib fimbrial gene cluster is located between the purE and pepN genes as previously described. For the 13 genital Haemophilusstrains that lack fimbrial genes, this region corresponds to a noncoding sequence. Another major fimbrial gene designated the fimbrin gene was previously identified in a nontypeable H. influenzae strain. A fimbrin-like gene was identified for all of our 19 genital strains. This gene is similar to the ompP5gene of many Haemophilus strains. Therefore, other, unidentified genes may explain the piliation observed in electron microscopy on genital Haemophilus strains which do not possess LKP-like fimbrial genes. Fimbrial genes were significantly associated with strains isolated from the genital tract. They may confer on the strain the ability to survive in the genital tract.
机译：研究了从生殖道感染（6株）和新生儿感染（13株）中分离出的属于嗜血杆菌属（emem）的一种遗传基因的19种分离株（在这里称为生殖株）的纤维基因。通过电子显微镜观察到十六个菌株表现出周生菌毛。通过使用对应于 b型流感嗜血杆菌（Hib） hifA 和 hifD 基因极端末端的引物进行PCR以及Southern印迹分析，在其中的6个中鉴定出了> hifA 样基因（名为 ghfA ）和 hifD 样基因（名为 ghfD ）。 19株。这六种菌株中有五种来自成人的生殖道，其中一株来自新生儿。对于每个基因，六个菌株的核苷酸序列相同。从嗜血杆菌的19个生殖器菌株中仅扩增出一个 hifE 样基因（名为 ghfE ），但 ghfE < / em>探针在与 ghfA 和 ghfD 呈阳性的其他五种菌株的Southern杂交中发出信号。因此，这些菌株可能携带 ghfE 样基因。如前所述，Hib纤维基因簇位于 purE 和 pepN 基因之间。对于13个缺乏纤维基因的生殖器 Haemophilus 菌株，该区域对应于一个非编码序列。先前在不可分型的 H中鉴定了另一种主要的纤维基因，称为纤维蛋白基因。流感菌株。我们所有的19个生殖器菌株都鉴定出了类似纤维蛋白的基因。该基因与许多嗜血杆菌菌株的 ompP5 基因相似。因此，其他未鉴定的基因可以解释在电子显微镜下观察到的生殖器嗜血杆菌菌株中不具有LKP样纤维基因的菌毛。纤维基因与从生殖道分离出的菌株显着相关。它们可以赋予菌株在生殖道中生存的能力。

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