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首页> 外文期刊>Infection and immunity >Fimbria-Mediated Enhanced Attachment of NontypeableHaemophilus influenzae to Respiratory Syncytial Virus-Infected Respiratory Epithelial Cells
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Fimbria-Mediated Enhanced Attachment of NontypeableHaemophilus influenzae to Respiratory Syncytial Virus-Infected Respiratory Epithelial Cells

机译：菌毛介导的不可分型流感嗜血杆菌与呼吸道合胞病毒感染的呼吸道上皮细胞的附着增强

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Respiratory syncytial virus (RSV) infection is known to predispose children to otitis media and sinusitis due to bacteria such as nontypeable Haemophilus influenzae (NTHI). In this study, we investigated the role of NTHI surface outer membrane protein P5-homologous fimbriae (P5-fimbriae) in attachment to RSV-exposed A549 epithelial cells. Analysis by fluorescence flow cytometry showed that a live P5-fimbriated NTHI strain (NTHIF+) attached to a higher proportion of RSV-exposed A549 cells than to control cells (mean, 68% for RSV versus 29% for control; P = 0.008), while attachment of the P5-fimbriae-deficient isogenic mutant strain (NTHIF?) was significantly lower than in control cells and rose only slightly following RSV exposure (mean, 17% for RSV versus 10% for control, P = 0.229). Attachment of NTHIF+ did not correlate with the amount of RSV antigen expressed by A549 cells. Furthermore, paraformaldehyde-fixed NTHIF+ also demonstrated an enhanced binding to RSV-exposed cells. Observations by transmission electronic microscopy showed that the mean number of bacteria attached per 100 RSV-exposed A549 cells was higher for NTHIF+ than NTHIF? (99 versus 18; P < 0.001). No intracellular bacteria were identified. UV-irradiated conditioned supernatants collected from RSV-infected A549 cultures (UV-cRSV) also enhanced the attachment of NTHIF+ to A549, suggesting the presence of a preformed soluble mediator(s) in UV-cRSV that enhances the expression of receptors for P5-fimbriae on A549 cells. In summary, RSV infection significantly enhances NTHI attachment to respiratory epithelial cells. P5-fimbria is the critical appendage of NTHI that participates in this attachment. In clinical settings, blocking of the P5-fimbria-mediated attachment of NTHIF+ by passive or active immunity may reduce the morbidity due to NTHI during RSV infection.
机译：众所周知，呼吸道合胞病毒（RSV）感染使儿童容易患中耳炎和鼻窦炎，原因是细菌是不可分型的流感嗜血杆菌（NTHI）。在这项研究中，我们调查了NTHI表面外膜蛋白P5同源菌毛（P5菌毛）在与RSV接触的A549上皮细胞附着中的作用。荧光流式细胞仪分析表明，与对照细胞相比，与RSV暴露的A549细胞相比，活的P5纤维化NTHI菌株（NTHI F + ）与比例较高的细胞连接（平均值，RSV为68％，而29％作为对照; P = 0.008），而缺乏P5-菌毛的同基因突变株（NTHI F？）的附着力显着低于对照细胞，仅略有上升RSV暴露后（平均值，RSV为17％，对照为10％， P = 0.229）。 NTHI F + 的附着与A549细胞表达的RSV抗原的数量无关。此外，低聚甲醛固定的NTHI F + 还显示出与暴露于RSV的细胞的结合增强。透射电子显微镜观察表明，NTHI F + 的每100个暴露于RSV的A549细胞附着的平均细菌数高于NTHI F？（99比18; P < 0.001）。没有发现细胞内细菌。从受RSV感染的A549培养物（UV-cRSV）收集的经过紫外线照射的条件上清液中，也增强了NTHI F + 对A549的附着，表明在UV-cRSV中存在预先形成的可溶性介体，增强A549细胞上P5-菌毛受体的表达。总之，RSV感染显着增强了NTHI对呼吸道上皮细胞的附着。 P5-菌毛是NTHI的重要附件。在临床环境中，被动或主动免疫阻止P5-菌毛介导的NTHI F + 附着可能会降低RSV感染期间NTHI的发病率。

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