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Biological Effects of Pseudomonas aeruginosa Type III-Secreted Proteins on CHO Cells

机译:铜绿假单胞菌III型分泌蛋白对CHO细胞的生物学效应

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A strain of Pseudomonas aeruginosa that fails to express known type III-secreted effector proteins was constructed as an expression host. Individual effectors were expressed intrans, and their biological effects on CHO cells were assessed in an acute cellular infection model. Intoxication with ExoS, ExoT, or ExoY resulted in alterations in cell morphology. As shown in previous genetic studies, ExoU expression was linked to acute cytotoxicity.
机译:构建不能表达已知的III型分泌型效应蛋白的铜绿假单胞菌菌株作为表达宿主。在 trans 中表达单个效应子,并在急性细胞感染模型中评估其对CHO细胞的生物学效应。 ExoS,ExoT或ExoY的中毒导致细胞形态发生改变。如先前的遗传研究所示,ExoU表达与急性细胞毒性有关。

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