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首页> 外文期刊>Infection and immunity >Protection against Candidiasis by an Immunoglobulin G3 (IgG3) Monoclonal Antibody Specific for the Same Mannotriose as an IgM Protective Antibody
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Protection against Candidiasis by an Immunoglobulin G3 (IgG3) Monoclonal Antibody Specific for the Same Mannotriose as an IgM Protective Antibody

机译：通过与IgM保护性抗体相同的甘露三糖特异性的免疫球蛋白G3（IgG3）单克隆抗体预防念珠菌病

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We previously reported that a liposome-mannan vaccine (L-mann) ofCandida albicans induces production of mouse antibodies that protect against disseminated candidiasis and vaginal infection. Immunoglobulin M (IgM) monoclonal antibody (MAb) B6.1, specific for aC. albicans cell surface β-1,2-mannotriose, protects mice against both infections. Another IgM MAb, termed B6, which is specific for a different cell surface mannan epitope, does not protect against disseminated candidiasis. The B6.1 epitope is displayed homogeneously over the entire cell surface, compared to a patchy distribution of the B6 epitope. To determine if protection is restricted to an IgM class of antibody, we tested an IgG antibody. MAb C3.1 was obtained from L-mann-immunized mice. By results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, capture enzyme-linked immunosorbent assay, and immunodiffusion tests, MAb C3.1 is an IgG3 isotype. By epitope inhibition assays, we determined that MAb C3.1 is specific for same mannotriose as MAb B6.1. As expected by the results of the inhibition assays, immunofluorescence microscopy showed that the C3.1 epitope is distributed on the yeast cell surface in a pattern identical to that of the B6.1 epitope. Kidney CFU and mean survival times of infected mice pretreated with MAb C3.1 indicated that the antibody enhanced resistance of mice against disseminated candidiasis. Mice in pseudoestrus that were given MAb C3.1 prior to vaginal infection developed fewer vaginal Candida CFU than control animals that received buffered saline instead of the antibody. The finding that an IgG3 antibody is protective is consistent with our hypothesis that epitope specificity and complement activation are related to the ability of an antibody to protect against candidiasis.
机译：我们先前曾报道白色念珠菌的脂质体-甘露聚糖疫苗（L-mann）诱导小鼠抗体的产生，该抗体可防止传播的念珠菌病和阴道感染。免疫球蛋白M（IgM）单克隆抗体（MAb）B6.1，对aC具有特异性。白色念珠菌细胞表面的β-1,2-甘露三糖可保护小鼠免受两种感染。另一种称为B6的IgM MAb，其对不同的细胞表面甘露聚糖表位具有特异性，不能防止散播的念珠菌病。与B6表位的斑片状分布相比，B6.1表位在整个细胞表面均匀显示。为了确定保护是否仅限于IgM类抗体，我们测试了IgG抗体。 MAb C3.1获自L-mann免疫的小鼠。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析，捕获酶联免疫吸附试验和免疫扩散试验的结果，MAb C3.1为IgG3同型。通过表位抑制试验，我们确定MAb C3.1对与MAb B6.1相同的甘露三糖具有特异性。如抑制试验结果所预期，免疫荧光显微镜检查显示C3.1表位以与B6.1表位相同的模式分布在酵母细胞表面。单克隆抗体C3.1预处理的受感染小鼠的肾脏CFU和平均存活时间表明，该抗体增强了小鼠对弥散性念珠菌病的抵抗力。在假发情期中，在阴道感染之前给予MAb C3.1的小鼠比接受缓冲盐水而不是抗体的对照动物产生更少的阴道念珠菌CFU。 IgG3抗体具有保护性的发现与我们的假设相符，即表位特异性和补体激活与抗体预防念珠菌病的能力有关。

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