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首页> 外文期刊>Infection and immunity >The Most Abundant Glycoprotein of Amebic Cyst Walls (Jacob) Is a Lectin with Five Cys-Rich, Chitin-Binding Domains
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The Most Abundant Glycoprotein of Amebic Cyst Walls (Jacob) Is a Lectin with Five Cys-Rich, Chitin-Binding Domains

机译：阿米巴囊肿壁（雅各布）中最丰富的糖蛋白是具有五个半胱氨酸，几丁质结合结构域的凝集素

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The infectious stage of amebae is the chitin-walled cyst, which is resistant to stomach acids. In this study an extraordinarily abundant, encystation-specific glycoprotein (Jacob) was identified on two-dimensional protein gels of cyst walls purified fromEntamoeba invadens. Jacob, which was acidic and had an apparent molecular mass of ～100 kDa, contained sugars that bound to concanavalin A and ricin. The jacob gene encoded a 45-kDa protein with a ladder-like series of five Cys-rich domains. These Cys-rich domains were reminiscent of but not homologous to the Cys-rich chitin-binding domains of insect chitinases and peritrophic matrix proteins that surround the food bolus in the insect gut. Jacob bound purified chitin and chitin remaining in sodium dodecyl sulfate-treated cyst walls. Conversely, the E. histolytica plasma membrane Gal/GalNAc lectin bound sugars of intact cyst walls and purified Jacob. In the presence of galactose, E. invadens formed wall-less cysts, which were quadranucleate and contained Jacob and chitinase (another encystation-specific protein) in secretory vesicles. A galactose lectin was found to be present on the surface of wall-less cysts, which phagocytosed bacteria and mucin-coated beads. These results suggest that the E. invadens cyst wall forms when the plasma membrane galactose lectin binds sugars on Jacob, which in turn binds chitin via its five chitin-binding domains.
机译：变形虫的感染期是几丁质壁囊肿，对胃酸有抵抗力。在这项研究中，从 Entamoeba invadens 纯化的囊壁的二维蛋白质凝胶上鉴定到了异常丰富的入侵特异性糖蛋白（Jacob）。雅各布呈酸性，表观分子量约为100 kDa，含有与伴刀豆球蛋白A和蓖麻毒蛋白结合的糖。 jacob 基因编码一个45 kDa的蛋白质，带有一个梯形序列，由五个富含Cys的结构域组成。这些富含Cys的结构域让人联想起昆虫几丁质酶和围绕昆虫肠道食物团的营养丰富的基质蛋白的富含Cys的几丁质结合结构域，但与它们不同源。雅各布结合纯化的几丁质和十二烷基硫酸钠处理过的囊壁中残留的几丁质。相反， E。组织溶质膜Gal / GalNAc凝集素结合完整囊肿壁的糖和纯化的Jacob。在半乳糖存在的情况下， E。入侵形成无壁囊肿，囊肿是四核的，分泌囊泡中含有雅各布和几丁质酶（另一种侵染特定蛋白）。发现无壁囊肿表面存在半乳糖凝集素，该囊肿吞噬了细菌和粘蛋白包被的珠子。这些结果表明 E。当质膜半乳糖凝集素结合雅各布上的糖时，其侵入囊壁，而雅各布上的糖又通过其五个几丁质结合域结合几丁质。

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