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首页> 外文期刊>Infection and immunity >Local Anticandidal Immune Responses in a Rat Model of Vaginal Infection by and Protection against Candida albicans
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Local Anticandidal Immune Responses in a Rat Model of Vaginal Infection by and Protection against Candida albicans

机译：在白色和白色念珠菌的阴道感染大鼠模型中的局部抗念珠菌免疫反应。

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Humoral (antibody [Ab]) and cellular Candida-specific immune responses in the vaginas of pseudoestrus rats were investigated during three successive infections by Candida albicans. After the first, protective infection, Abs against mannan and aspartyl proteinase antigens were present in the vaginal fluid, and their titers clearly increased during the two subsequent, rapidly healing infections. In all animals, about 65 and 10% of vaginal lymphocytes (VL) were CD3+ (T cells) and CD3?CD5+ (B cells), respectively. Two-thirds of the CD3+ T cells expressed the α/β and one-third expressed the γ/δ T-cell receptor (TCR). This proportion slightly fluctuated during the three rounds of C. albicans infection, but no significant differences between infected and noninfected rats were found. More relevant were the changes in the CD4+/CD8+ T-cell ratio, particularly for cells bearing the CD25 (interleukin-2 receptor α) marker. In fact, a progressively increased number of both CD4+ α/β TCR and CD4+ CD25+ VL was observed after the second and third Candida challenges, reversing the high initial CD8+ cell number of controls (estrogenized but uninfected rats). The CD3? CD5+ cells also almost doubled from the first to the third infection. Analysis of the cytokines secreted in the vaginal fluid of Candida-infected rats showed high levels of interleukin 12 (IL-12) during the first infection, followed by progressively increasing amounts of IL-2 and gamma interferon during the subsequent infections. No IL-4 or IL-5 was ever detected. During the third infection, VL with in vitro proliferative activity in response to an immunodominant mannoprotein antigen of C. albicans were present in the vaginal tissue. No response to this antigen by mitogen-responsive blood, lymph node, and spleen cells was found. In summary, the presence of protective Ab and T helper type 1 cytokines in the vaginal fluids, the in vitro proliferation of vaginal lymphocytes in response toCandida antigenic stimulation, and the increased number of activated CD4+ cells and some special B lymphocytes afterC. albicans challenge constitute good evidence for induction of locally expressed Candida-specific Ab and cellular responses which are potentially involved in anticandidal protection at the vaginal level.
机译：在白色念珠菌连续感染三次期间，研究了假发情期大鼠阴道中的体液（抗体[Ab]）和细胞念珠菌特异性免疫反应。在第一次保护性感染后，阴道液中存在抗甘露聚糖和天冬氨酰蛋白酶抗原的抗体，在随后的两次快速治愈的感染中，它们的滴度明显增加。在所有动物中，约65％和10％的阴道淋巴细胞（VL）是CD3 +（T细胞）和CD3？CD5 +（B细胞）。三分之二的CD3 + T细胞表达α/β，三分之一表达γ/δT细胞受体（TCR）。在三轮白色念珠菌感染期间，该比例略有波动，但在感染和未感染的大鼠之间未发现显着差异。更相关的是CD4 + / CD8 + T细胞比率的变化，特别是对于带有CD25（白介素2受体α）标记的细胞。实际上，在第二次和第三次念珠菌攻击后，观察到CD4 +α/βTCR和CD4 + CD25 + VL的数量逐渐增加，从而逆转了对照（经雌性但未感染的大鼠）的初始CD8 +细胞数量较高的现象。 CD3？从第一次感染到第三次感染，CD5 +细胞也几乎翻了一番。对念珠菌感染大鼠阴道液中分泌的细胞因子的分析显示，在初次感染期间白细胞介素12（IL-12）含量高，随后在随后的感染期间逐渐增加IL-2和γ干扰素的含量。从未检测到IL-4或IL-5。在第三次感染期间，阴道组织中存在对白念珠菌的免疫占主导地位的甘露糖蛋白抗原具有体外增殖活性的VL。未发现丝裂原反应性血液，淋巴结和脾细胞对此抗原有反应。总之，阴道液中存在保护性Ab和T辅助1型细胞因子，响应于Candida抗原刺激的阴道淋巴细胞体外增殖，以及C后活化的CD4 +细胞和某些特殊的B淋巴细胞数量增加。白色念珠菌攻击构成了诱导局部表达的念珠菌特异性抗体和细胞反应的良好证据，这些抗原可能在阴道水平上参与抗念珠菌的保护。

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