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首页> 外文期刊>Infection and immunity >Neisseria meningitidisLipopolysaccharide Modulates the Specific Humoral Immune Response to Neisserial Porins but Has No Effect on Porin-Induced Upregulation of Costimulatory Ligand B7-2
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Neisseria meningitidisLipopolysaccharide Modulates the Specific Humoral Immune Response to Neisserial Porins but Has No Effect on Porin-Induced Upregulation of Costimulatory Ligand B7-2

机译：脑膜炎奈瑟氏球菌脂多糖调节对奈瑟氏菌孔蛋白的特定体液免疫反应，但对孔蛋白诱导的共刺激配体B7-2上调没有影响。

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The role of lipopolysaccharide (LPS) in the specific humoral response to meningococcal porins was investigated by measuring anti-PorA or -PorB antibody levels in mice immunized with wild-type meningococcal strain H44/76 or with its recently described LPS-negative mutant. Two murine strains were used for these immunizations: C3H/HeJ, which is LPS hyporesponsive, or C3H/HeOuJ, which is LPS responsive. A high level of anti-PorB immunoglobulin G (IgG) response was induced in both strains of mice immunized with either organism. The response induced by the wild-type strain was greater in C3H/HeOuJ mice than in C3H/HeJ mice, while the response induced by the LPS-negative mutant was similar in the two murine strains. Additionally, the anti-PorB response was similar in C3H/HeJ mice immunized with either bacterial strain. In general, the anti-PorA IgG response was lower than the anti-PorB response. These findings indicate that the presence of LPS is not essential for the induction of an antineisserial porin humoral response but can augment such a response. To determine whether LPS has any effect on the B-cell-stimulatory effect of neisserial porins (essential for the adjuvant activity of neisserial porins), B cells from both murine strains were incubated with outer membrane complexes (OMCs) prepared from strain H44/76 and its LPS-negative mutant. OMCs from either meningococcal strain were able to increase the surface expression of the costimulatory ligand B7-2 on B cells from either murine strain. Consistent with previously reported findings, LPS does not significantly affect the ability of neisserial porins to induce the costimulatory ligand B7-2.
机译：通过测量用野生型脑膜炎球菌菌株H44 / 76或其最近描述的LPS阴性突变体免疫的小鼠中的抗PorA或-PorB抗体水平，研究了脂多糖（LPS）在对脑膜炎球菌孔蛋白的特定体液应答中的作用。使用两种鼠类疫苗进行免疫：C3H / HeJ（对LPS应答低）或C3H / HeOuJ（对LPS应答）。在用任何一种生物体免疫的两种小鼠中都诱导出高水平的抗PorB免疫球蛋白G（IgG）反应。在C3H / HeOuJ小鼠中，野生型菌株诱导的应答比在C3H / HeJ小鼠中更大，而在两个鼠类菌株中，LPS阴性突变体诱导的应答相似。另外，在用任一细菌菌株免疫的C3H / HeJ小鼠中，抗PorB反应相似。通常，抗PorA IgG应答低于抗PorB应答。这些发现表明LPS的存在对于诱导抗前体孔蛋白体液反应不是必需的，但是可以增强这种反应。为了确定LPS是否对奈瑟氏菌孔蛋白的B细胞刺激作用（对奈瑟氏菌孔蛋白的佐剂活性必不可少）有影响，将两种鼠类菌株的B细胞与由菌株H44 / 76制备的外膜复合物（OMC）一起孵育及其LPS阴性突变体。来自任何一个脑膜炎球菌菌株的OMC都能够增加任何一个小鼠菌株的B细胞上共刺激配体B7-2的表面表达。与先前报道的发现一致，LPS不会显着影响奈瑟氏菌孔蛋白诱导共刺激配体B7-2的能力。

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