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Orally Administered Giardia duodenalis Extracts Enhance an Antigen-Specific Antibody Response

机译：口服Giardia duodenalis提取物可增强抗原特异性抗体反应

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We have identified novel adjuvant activity in specific cytosol fractions from trophozoites of Giardia isolate BRIS/95/HEPU/2041 (J. A. Upcroft, P. A. McDonnell, and P. Upcroft, Parasitol. Today, 14:281–284, 1998). Adjuvant activity was demonstrated in the systemic and mucosal compartments when Giardia extract was coadministered orally with antigen to mice. Enhanced antigen-specific serum antibody responses were demonstrated by enzyme-linked immunosorbent assay to be comparable to those generated by the “gold standard,” mucosal adjuvant cholera toxin. A source of adjuvant activity was localized to the cytosolic component of the parasite. Fractionation of the cytosol produced fraction pools, some of which, when coadministered with antigen, stimulated an enhanced antigen-specific serum response. The toxic component of conventional mucosal adjuvants is associated with adjuvant activity; therefore, in a similar way, the toxin-like attributes of BRIS/95/HEPU/2041 may be responsible for its adjuvanticity. Complete characterization of the adjuvant is under way.
机译：我们从贾第鞭毛虫的滋养体中分离出BRIS / 95 / HEPU / 2041（JA Upcroft，PA McDonnell和P. Upcroft，Parasitol。）的特定胞质组分中发现了新的佐剂活性。今天，14：281–284 （1998年）。将提取物与抗原一起口服给予小鼠时，在全身和粘膜区室均显示出佐剂活性。酶联免疫吸附试验证明增强的抗原特异性血清抗体应答与“金标准”（粘膜佐剂霍乱毒素）产生的应答相当。佐剂活性的来源位于该寄生虫的胞质成分中。溶质的分级分离产生了级分库，其中一些与抗原共同施用可刺激增强的抗原特异性血清反应。常规粘膜佐剂的毒性成分与佐剂活性有关。因此，以类似的方式，BRIS / 95 / HEPU / 2041的毒素样属性可能是其辅助作用的原因。佐剂的完整表征正在进行中。

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