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首页> 外文期刊>Infection and immunity >Role of Streptococcus gordoniiAmylase-Binding Protein A in Adhesion to Hydroxyapatite, Starch Metabolism, and Biofilm Formation
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Role of Streptococcus gordoniiAmylase-Binding Protein A in Adhesion to Hydroxyapatite, Starch Metabolism, and Biofilm Formation

机译：戈登链球菌淀粉酶结合蛋白A在粘附羟基磷灰石，淀粉代谢和生物膜形成中的作用

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Interactions between bacteria and salivary components are thought to be important in the establishment and ecology of the oral microflora. α-Amylase, the predominant salivary enzyme in humans, binds to Streptococcus gordonii, a primary colonizer of the tooth. Previous studies have implicated this interaction in adhesion of the bacteria to salivary pellicles, catabolism of dietary starches, and biofilm formation. Amylase binding is mediated at least in part by the amylase-binding protein A (AbpA). To study the function of this protein, an erythromycin resistance determinant [erm(AM)] was inserted within the abpAgene of S. gordonii strains Challis and FAS4 by allelic exchange, resulting in abpA mutant strains Challis-E1 and FAS4-E1. Comparison of the wild-type and mutant strains did not reveal any significant differences in colony morphology, biochemical metabolic profiles, growth in complex or defined media, surface hydrophobicity, or coaggregation properties. Scatchard analysis of adhesion isotherms demonstrated that the wild-type strains adhered better to human parotid-saliva- and amylase-coated hydroxyapatite than did the AbpA mutants. In contrast, the mutant strains bound to whole-saliva-coated hydroxyapatite to a greater extent than did the wild-type strains. While the wild-type strains preincubated with purified salivary amylase grew well in defined medium with potato starch as the sole carbohydrate source, the AbpA mutants did not grow under the same conditions even after preincubation with amylase. In addition, the wild-type strain produced large microcolonies in a flow cell biofilm model, while the abpA mutant strains grew much more poorly and produced relatively small microcolonies. Taken together, these results suggest that AbpA ofS. gordonii functions as an adhesin to amylase-coated hydroxyapatite, in salivary-amylase-mediated catabolism of dietary starches and in human saliva-supported biofilm formation by S. gordonii.
机译：细菌和唾液成分之间的相互作用被认为对口腔微生物区系的建立和生态学很重要。 α-淀粉酶是人类最主要的唾液酶，它与牙齿的主要定居者 Streptococcus gordonii 结合。先前的研究已暗示这种相互作用与细菌与唾液薄膜的粘附，饮食淀粉的分解代谢和生物膜形成有关。淀粉酶结合至少部分地由淀粉酶结合蛋白A（AbpA）介导。为了研究该蛋白的功能，将红霉素抗性决定簇[ erm （AM）]插入 S的 abpA 基因中。 gordonii 菌株Challis和FAS4通过等位基因交换产生 abpA 突变株Challis-E1和FAS4-E1。野生型和突变型菌株的比较未发现菌落形态，生化代谢谱，复杂或确定培养基中的生长，表面疏水性或共聚集特性有任何显着差异。粘附等温线的Scatchard分析表明，与AbpA突变体相比，野生型菌株对人腮腺和淀粉酶包被的羟基磷灰石的粘附更好。相反，与野生型菌株相比，突变菌株与全唾液包被的羟基磷灰石结合的程度更大。尽管用纯化唾液淀粉酶预孵育的野生型菌株在以马铃薯淀粉为唯一碳水化合物源的确定培养基中生长良好，但即使在用淀粉酶预孵育后，AbpA突变体也无法在相同条件下生长。此外，野生型菌株在流动细胞生物膜模型中产生大的微克隆，而 abpA 突变菌株生长更差并且产生相对小的微克隆。综上，这些结果表明 S的AbpA。 gordonii 在淀粉酶包覆的羟磷灰石，唾液淀粉酶介导的饮食淀粉分解代谢以及人类唾液支持的 S生物膜形成中起粘附剂的作用。戈登。

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