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首页> 外文期刊>Infection and immunity >Phenotypic and Genomic Analyses of theMycobacterium avium Complex Reveal Differences in Gastrointestinal Invasion and Genomic Composition
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Phenotypic and Genomic Analyses of theMycobacterium avium Complex Reveal Differences in Gastrointestinal Invasion and Genomic Composition

机译：鸟分枝杆菌复合体的表型和基因组分析揭示了胃肠道侵袭和基因组组成的差异

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Mycobacterium avium and Mycobacterium intracellulare are closely related organisms and comprise theMycobacterium avium complex. These organisms share many common characteristics, including the ability to cause life-threatening respiratory infections in people with underlying lung pathology or immunological defects and occasionally in those with no known predisposing conditions. However, the ability to invade the mucosa of the gastrointestinal tract and cause disseminated disease in AIDS patients has not been epidemiologically linked to M. intracellulare and appears to be unique to M. avium. We compared the abilities of M. avium andM. intracellulare to tolerate the acidic conditions of the stomach, to resist the membrane-disrupting activity of cationic peptides, and to invade intestinal epithelial cells in vitro and in vivo. We observed that M. avium and M. intracellulare were both tolerant to the acidic conditions encountered in the stomach and resistant to cationic peptides. However, when strains of M. avium and M. intracellulare were examined for their ability to enter cultured human intestinal cells or mouse intestinal mucosa, we observed that M. avium could invade more efficiently thanM. intracellulare. To elucidate the basis of this pathogenic difference and identify genes involved in the invasion of the intestinal mucosa, we performed chromosomal DNA subtractive hybridization using M. avium and M. intracellulare chromosomal DNAs. In all, 21 genes that were present in M. avium but absent in M. intracellulare were identified, including some that may be associated with the ability of M. avium to invade the intestinal mucosa.
机译：禽分枝杆菌和胞内分枝杆菌是密切相关的生物，由禽分枝杆菌复合物组成。这些生物具有许多共同的特征，包括在具有潜在的肺部病理或免疫缺陷的人中，甚至在没有已知易感性疾病的人中，引起致命的呼吸道感染的能力。但是，侵袭胃肠道粘膜并引起AIDS患者传播疾病的能力尚未在流行病学上与 M相关。细胞内，并且似乎对 M是唯一的。 avium 。我们比较了 M的能力。鸟卵和 M。细胞内耐受胃的酸性条件，抵抗阳离子肽的膜破坏活性，并在体内和体外侵袭肠上皮细胞。我们观察到 M。 avium 和 M。细胞内细胞既能耐受胃中遇到的酸性条件又能抵抗阳离子肽。但是，当 M菌株时。 avium 和 M。检查细胞内进入培养的人肠道细胞或小鼠肠道粘膜的能力，我们观察到 M。鸟比 M更有效地入侵。细胞内。为了阐明这种病原性差异的基础并鉴定参与肠粘膜侵袭的基因，我们使用 M进行了染色体DNA消减杂交。 avium 和 M。细胞内染色体DNA共有21种基因存在于 M中。鸟卵，但在 M中不存在。鉴定出细胞内，包括一些可能与 M能力有关的细胞。鸟卵侵入肠粘膜。

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