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首页> 外文期刊>Infection and immunity >Identification and Characterization of a New Staphylococcal Enterotoxin-Related Putative Toxin Encoded by Two Kinds of Plasmids
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Identification and Characterization of a New Staphylococcal Enterotoxin-Related Putative Toxin Encoded by Two Kinds of Plasmids

机译：两种质粒编码的新型葡萄球菌肠毒素相关推定毒素的鉴定与表征

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We identified and characterized a novel staphylococcal enterotoxin-like putative toxin, which is named SER. Nucleotide sequencing analysis of the ser gene revealed that ser was most closely related to the seg gene. The ser gene product, SER, was successfully expressed as a recombinant protein in an Escherichia coli expression system, and recombinant SER (rSER) showed significant T-cell stimulation activity. The SER production in ser-harboring Staphylococcus aureus strains was confirmed by Western blot analysis using anti-rSER antibody. Moreover, ser was seen to be encoded by at least two types of plasmids. In particular, one kind of plasmid encoding the ser gene has been known as a sed- and sej-carrying pIB485-related plasmid.
机译：我们确定并表征了一种新型的葡萄球菌肠毒素样推定毒素，称为SER。对 ser 基因的核苷酸测序分析表明， ser 与 seg 基因关系最密切。 ser 基因产物SER在大肠杆菌表达系统中成功表达为重组蛋白，重组SER（rSER）显示出显着的T细胞刺激活性。使用抗rSER抗体的Western印迹分析证实了携带 ser 的金黄色葡萄球菌菌株的SER产生。此外，发现 ser 由至少两种类型的质粒编码。特别地，已知一种编码 ser 基因的质粒是 sed -和 sej 携带的pIB485相关质粒。

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