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首页> 外文期刊>Infection and immunity >Porphyromonas gingivalis Infection in Pregnant Mice Is Associated with Placental Dissemination, an Increase in the Placental Th1/Th2 Cytokine Ratio, and Fetal Growth Restriction
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Porphyromonas gingivalis Infection in Pregnant Mice Is Associated with Placental Dissemination, an Increase in the Placental Th1/Th2 Cytokine Ratio, and Fetal Growth Restriction

机译：孕妇小鼠牙龈卟啉单胞菌感染与胎盘播散，胎盘Th1 / Th2细胞因子比率增加和胎儿生长受限有关

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Our previous animal studies showed that maternal Porphyromonas gingivalis infection in a subcutaneous chamber is associated with hepatic and uterine translocation, as well as systemic induction of maternal inflammatory responses, both of which were associated with fetal growth restriction (FGR). However, P. gingivalis-challenged dams had fetuses with either FGR (2 standard deviations below mean weight of nonchallenged dams) or normal weight. Therefore, the objective of this study was to determine whether maternal infection with P. gingivalis compromises normal fetal development via direct placental invasion and induction of fetus-specific placental immune responses characterized by a proinflammatory Th1-type cytokine profile. P. gingivalis-specific DNA was detected in placentas and fetuses of FGR and normal littermates from P. gingivalis-infected dams. Th1- and Th2-type cytokine mRNA as well as tumor necrosis factor alpha and transforming growth factor β2 mRNA were examined in placental tissue by using reverse transcription-PCR to determine Th1/Th2 ratios. For eight litters containing both normal-weight and FGR fetuses, P. gingivalis DNA was detected only in the placentas of FGR fetuses. All fetuses and all amniotic fluid samples from infected and control dams were negative for P. gingivalis DNA. mRNA levels of gamma interferon and interleukin-2 (IL-2) were significantly increased in placentas of FGR fetuses, while expression of IL-10 was significantly decreased in the same group. These data indicate that, in P. gingivalis-challenged dams, within each litter there is placenta-specific translocation of P. gingivalis that results in growth restriction of the targeted fetus, which is associated with a shift in the placental Th1/Th2 cytokine balance.
机译：我们以前的动物研究表明，皮下腔内的母亲 Porphyromonas gingivalis 感染与肝和子宫易位以及母亲炎症反应的系统性诱导有关，这两者均与胎儿生长受限（FGR）相关）。但是， P。牙龈受挑战的大坝的胎儿具有FGR（低于非挑战水坝的平均体重2个标准差）或正常体重。因此，本研究的目的是确定产妇是否感染了 P。牙龈炎通过直接的胎盘浸润和诱导以促炎性Th1型细胞因子谱为特征的胎儿特异性胎盘免疫应答而损害正常的胎儿发育。 P。在FGR的胎盘和胎儿以及 P的正常同窝仔中检测到牙龈特异性DNA。牙龈感染的水坝。通过使用逆转录PCR确定胎盘组织中Th1 / Th2的比率，对Th1-和Th2型细胞因子mRNA以及肿瘤坏死因子α和转化生长因子β2mRNA进行了检查。对于同时含有正常体重和FGR胎儿的八胎， P。仅在FGR胎儿的胎盘中检测到牙龈DNA。来自感染大坝和对照大坝的所有胎儿和所有羊水样本的 P均为阴性。牙龈DNA。 FGR胎儿胎盘中γ干扰素和白介素2（IL-2）的mRNA水平显着升高，而同一组中IL-10的表达显着降低。这些数据表明在 P中。牙龈病攻击的水坝，在每个垫料中都有 P的胎盘特定易位。牙龈炎导致目标胎儿的生长受限，这与胎盘Th1 / Th2细胞因子平衡的改变有关。

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