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首页> 外文期刊>Infection and immunity >A Salmonella fim Homologue in Citrobacter freundii Mediates Invasion In Vitro and Crossing of the Blood-Brain Barrier in the Rat Pup Model
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A Salmonella fim Homologue in Citrobacter freundii Mediates Invasion In Vitro and Crossing of the Blood-Brain Barrier in the Rat Pup Model

机译：弗氏柠檬酸杆菌中的沙门氏菌同源性介导了大鼠幼犬模型的体外侵袭和血脑屏障的交叉。

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From the invasive Citrobacter freundii strain 3009, an invasion determinant was cloned, sequenced, and expressed. Sequence analysis of the determinant showed high homology with the fim determinant from Salmonella enterica serovar Typhimurium. The genes of the invasion determinant directed invasion of recombinant Escherichia coli K-12 strains into human epithelial cell lines of the bladder and gut as well as mannose-sensitive yeast agglutination and were termed fimCf genes. Expression of the FimCf proteins was shown by 35S labeling and/or Western blotting. In the infant rat model of experimental hematogenous meningitis, C. freundii strain 3009 and the in vitro invasive recombinant E. coli K-12 strain harboring the fimCf determinant reached the cerebrospinal fluid, in contrast to the case for the control strain. The fim determinant was also necessary for efficient in vitro invasion by C. freundii, because a deletion mutant was strongly reduced in its invasion efficiency. The mutation could be complemented in trans by the corresponding genes. Invasion by C. freundii could be blocked only by d-mannose, GlcNAc, and chitin hydrolysate and not by other carbohydrates tested. In contrast, yeast agglutination was not affected by GlcNAc or chitin hydrolysate. This finding indicated mannose residues to be essential for both yeast agglutination and invasion, whereas GlcNAc (oligomer) residues of host cells are involved exclusively in invasion. These results showed the fim determinant of C. freundii to be responsible for d-mannose- and GlcNAc-dependent in vitro invasion without being assembled into pili and for crossing of the blood-brain barrier in the infant rat model.
机译：从侵袭性弗氏柠檬酸杆菌3009菌株中克隆侵袭决定簇，测序并表达。该决定簇的序列分析与 Salmonella enterica 鼠伤寒沙门氏菌 fim 决定簇具有高度同源性。侵袭决定因子基因将重组大肠杆菌 K-12菌株定向侵袭到人的膀胱和肠道上皮细胞系以及甘露糖敏感的酵母凝集物中，被称为 fim 。 em> Cf 基因。 Fim Cf 蛋白的表达通过 35 S标记和/或Western blotting显示。在实验性血源性脑膜炎的幼鼠模型中， C。 freundii 菌株3009和体外侵入性重组 E。带有 fim Cf 决定簇的大肠杆菌 K-12菌株到达脑脊液，与对照菌株相反。 fim 决定簇对于 C的有效体外入侵也是必需的。 freundii ，因为缺失突变体的入侵效率大大降低。该突变可以由相应的基因在 trans 中进行补充。 C的入侵。只能用d-甘露糖，GlcNAc和几丁质水解物来阻止弗氏，而不是通过其他测试的碳水化合物来阻止。相反，酵母凝集不受GlcNAc或几丁质水解物的影响。该发现表明甘露糖残基对于酵母凝集和侵袭都是必不可少的，而宿主细胞的GlcNAc（寡聚物）残基仅与侵袭有关。这些结果表明 C的 fim 决定因素。 freundii 负责d-甘露糖和GlcNAc依赖的体外侵袭，而无需组装成菌毛，也不会在婴儿大鼠模型中穿越血脑屏障。

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