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首页> 外文期刊>Infection and immunity >Cholesterol metabolism by Treponema hyodysenteriae.
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Cholesterol metabolism by Treponema hyodysenteriae.

机译：猪痢疾密螺旋体的胆固醇代谢。

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The sterol content of cellular lipids of Treponema hyodysenteriae, the agent of swine dysentery, was determined. When cultured in lipid-depleted brain heart infusion broth containing vesicles made from [4-14C]cholesterol and phosphatidylcholine, T. hyodysenteriae cells incorporated radioactive label. Most (95%) of this radioactivity was associated with bacterial membrane preparations. Lipids were extracted from radiolabeled cells and fractionated by silicic acid column chromatography. Components of the neutral lipid fraction were separated by reversed-phase high-performance liquid chromatography and were detected by monitoring both radioactivity and UV absorption (210 nm) of the column effluent. Cholesterol represented only about 5% of the total radioactivity in the bacterial neutral lipids. The remaining radioactivity was associated with a compound that did not absorb light at 210 nm. This lipid was purified and, on the basis of results from thin-layer chromatography and mass spectrometry, was identified as cholesterol (5 alpha-cholestan-3 beta-ol), a sterol lacking the unsaturated bond of cholesterol. Cholestanol was also present in cell-free culture broth, but only after growth of the spirochete. These results are evidence that cholesterol is used by T. hyodysenteriae for membrane synthesis. Cholesterol is converted to cholestanol in T. hyodysenteriae cultures and cholestanol is a major component (approximately 9% by weight) of T. hyodysenteriae cell lipids.
机译：确定猪痢疾密螺旋体，猪痢疾密螺旋体的细胞脂质的甾醇含量。当在含有[4-14C]胆固醇和磷脂酰胆碱制成的囊泡的贫脂质脑心浸液中培养时，猪痢疾短螺旋体细胞掺入了放射性标记。这种放射性的大部分（95％）与细菌膜的制备有关。从放射性标记的细胞中提取脂质，并通过硅酸柱色谱分离。中性脂质级分的成分通过反相高效液相色谱分离，并通过监测柱流出物的放射性和紫外线吸收（210 nm）进行检测。在细菌中性脂质中，胆固醇仅占总放射性的约5％。剩余的放射性与在210 nm处不吸收光的化合物有关。纯化该脂质，并根据薄层色谱和质谱的结果，将其鉴定为胆固醇（5α-胆甾烷-3β-醇），这是一种缺乏胆固醇不饱和键的固醇。胆固醇也存在于无细胞培养液中，但仅在螺旋体生长后才存在。这些结果证明猪痢疾短螺旋体将胆固醇用于膜合成。在猪痢疾短螺旋体培养物中，胆固醇被转化为胆固醇，并且胆固醇是猪痢疾短螺旋体细胞脂质的主要成分（按重量计约9％）。

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