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首页> 外文期刊>Infection and immunity >Naturally occurring antibodies to an epitope on Plasmodium falciparum gametes detected by monoclonal antibody-based competitive enzyme-linked immunosorbent assay.
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Naturally occurring antibodies to an epitope on Plasmodium falciparum gametes detected by monoclonal antibody-based competitive enzyme-linked immunosorbent assay.

机译：通过基于单克隆抗体的竞争性酶联免疫吸附测定法检测到的恶性疟原虫配子表位的天然存在抗体。

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摘要

The antibody response to an epitope on gamete antigens of Plasmodium falciparum in persons naturally exposed to malaria has been investigated by competitive enzyme-linked immunosorbent assay. The assay detects antibodies to an epitope on the 48/45-kilodalton (kDa) gamete surface antigen by competition with horseradish peroxidase-labeled monoclonal antibody IIC5-B10. Five sera previously shown to immunoprecipitate the 230- and 48/45-kDa antigens significantly inhibited IIC5-B10 binding to an average of 24.2% of control. The one serum which precipitated only the 48/45-kDa antigen did not inhibit IIC5-B10 binding. For 26 sera which were negative by immunoprecipitation, mean binding in the assay was 112.7% of control (pooled London nonimmune sera). Recognition of both 230-kDa and 48/45-kDa antigens was associated with a titer of 1:9 or greater (reciprocal geometric mean titer, 27.6) for inhibition to more than 2 standard deviations from the mean of the negative sera. The results show that the IIC5-B10 binding site is a naturally immunogenic epitope recognized by the majority of persons who had antibodies to the 48/45-kDa protein. An additional finding was enhancement of binding of IIC5-B10 to an average of 154.4% of control by five sera which recognized only the 230-kDa antigen, presumably due to conformational alteration of the gamete antigen complex.
机译：已经通过竞争性酶联免疫吸附试验研究了自然暴露于疟疾的人对恶性疟原虫配子抗原表位的抗体反应。该测定法通过与辣根过氧化物酶标记的单克隆抗体IIC5-B10竞争来检测针对48 / 45-千达尔顿（kDa）配子表面抗原上表位的抗体。先前显示可免疫沉淀230-和48 / 45-kDa抗原的5个血清显着抑制IIC5-B10的结合，平均达到对照的24.2％。仅沉淀48 / 45-kDa抗原的一种血清不抑制IIC5-B10结合。对于通过免疫沉淀呈阴性的26个血清，测定中的平均结合率为对照的112.7％（合并的伦敦非免疫血清）。 230-kDa和48 / 45-kDa抗原的识别均与1：9或更高的滴度相关（几何平均滴度为27.6），以抑制从阴性血清均值的2个以上标准偏差。结果表明，IIC5-B10结合位点是天然免疫原性表位，大多数人都具有针对48 / 45-kDa蛋白的抗体。另一个发现是IIC5-B10与仅识别230 kDa抗原的五个血清的IIC5-B10结合平均提高了154.4％的对照，大概是由于配子抗原复合物的构象改变。

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