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首页> 外文期刊>Infection and immunity >Immunoreactivity of neoglycolipids constructed from oligomannosidic residues of the Candida albicans cell wall.
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Immunoreactivity of neoglycolipids constructed from oligomannosidic residues of the Candida albicans cell wall.

机译：由白色念珠菌细胞壁的寡甘露糖苷残基构建的新糖脂的免疫反应性。

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To establish a model to study the immunoreactivity of oligosaccharidic structures from the Candida albicans cell wall, we attempted to construct neoglycolipids with these residues by using oligomannosides released after mild acid hydrolysis of the phosphopeptidomannans isolated from yeast forms. From a mixture of manno-oligosaccharides ranging from mannobiose to mannononaose, the structure of a quantitatively major component (mannotriose) was determined to be Man (beta 1-2) Man (beta 1-2) Man alpha by 1H nuclear magnetic resonance analysis. After coupling of the pool of oligosaccharides to a lipid (4-hexadecylaniline), the synthesized molecules were injected into mice and rats. Antibody responses were detected on enzyme-linked immunosorbent assay plates coated with either phosphopeptidomannans or neoglycolipids. The hybrid molecules exhibited both immunogenicity and antigenicity. The kinetics of antibody responses as well as immunofluorescence patterns observed on whole C. albicans cells strongly mimicked results from the immunization of animals with natural antigens. Construction of neoglycolipids could therefore provide an interesting approach to the study of specific oligosaccharides of C. albicans and their recognition by the host immune system.
机译：为了建立模型来研究来自白色念珠菌细胞壁的寡糖结构的免疫反应性，我们试图通过使用从酵母形式中分离出的磷酸肽甘露聚糖的弱酸水解后释放的寡甘露糖苷来构建具有这些残基的新糖脂。从甘露二糖到甘露核糖的甘露寡糖混合物中，通过1H核磁共振分析确定了定量主要成分（甘露三糖）的结构为Man（beta 1-2）Man（beta 1-2）Man alpha。在寡糖库与脂质（4-十六烷基苯胺）偶联后，将合成的分子注射入小鼠和大鼠。在涂有磷酸肽甘露聚糖或新糖脂的酶联免疫吸附测定板上检测抗体应答。杂种分子表现出免疫原性和抗原性。用天然抗原免疫动物后，在整个白色念珠菌细胞上观察到的抗体反应动力学和免疫荧光模式被强烈模仿。因此，新糖脂的构建可以为研究白色念珠菌的特定寡糖及其被宿主免疫系统识别提供有趣的方法。

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