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首页> 外文期刊>Infection and immunity >Identification of subregions of Bordetella pertussis filamentous hemagglutinin that stimulate human T-cell responses.
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Identification of subregions of Bordetella pertussis filamentous hemagglutinin that stimulate human T-cell responses.

机译：鉴定刺激人T细胞反应的百日咳博德特氏菌丝状血凝素子区域。

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摘要

Filamentous hemagglutinin (FHA), a 220-kDa protein that mediates the adhesion of Bordetella pertussis to eukaryotic cells, is a component of acellular vaccines against whooping cough. To identify the subregions of FHA that are immunogenic for T cells, 16 human T-cell clones were raised against purified FHA and tested for the recognition of recombinant and proteolytic fragments. The clones were found to map either in the carboxy-terminal or the amino-terminal part of the FHA molecule, but none of them recognized the central region, which contains a sequence that is homologous to that of the eukaryotic protein fibronectin. These data suggest that subregions of FHA that do not contain sequences that are potentially cross-reactive with self proteins may be sufficient to induce an immune response against the whole protein.
机译：丝状血凝素（FHA）是一种220 kDa的蛋白质，介导百日咳博德特氏菌与真核细胞的粘附，是抗百日咳的脱细胞疫苗的组成部分。为了鉴定对T细胞具有免疫原性的FHA子区域，针对纯化的FHA产生了16个人类T细胞克隆，并测试了其对重组和蛋白水解片段的识别。发现该克隆在FHA分子的羧基末端或氨基末端部分定位，但它们均未识别出中央区域，该中央区域包含与真核蛋白纤连蛋白同源的序列。这些数据表明，不包含可能与自身蛋白质发生交叉反应的序列的FHA子区域可能足以诱导针对整个蛋白质的免疫应答。

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