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首页> 外文期刊>Infection and immunity >New model for analysis of mucosal immunity: intestinal secretion of specific monoclonal immunoglobulin A from hybridoma tumors protects against Vibrio cholerae infection.
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New model for analysis of mucosal immunity: intestinal secretion of specific monoclonal immunoglobulin A from hybridoma tumors protects against Vibrio cholerae infection.

机译：用于分析粘膜免疫力的新模型：杂交瘤肿瘤中特定单克隆免疫球蛋白A的肠道分泌可防止霍乱弧菌感染。

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摘要

Secretory immunoglobulin A (sIgA) plays a role in defense against Vibrio cholerae and other microorganisms that infect mucosal surfaces, but it is not established whether sIgA alone can prevent disease. We report here a strategy for identifying the antigen specificities of monoclonal sIgA antibodies that are capable of providing such protection. IgA hybridomas were generated from Peyer's patch lymphocytes after oral immunization with V. cholerae Ogawa 395. A clone was selected that produced dimeric monoclonal IgA antibodies directed against an Ogawa-specific lipopolysaccharide carbohydrate antigen exposed on the bacterial surface. Hybridoma cells were used to produce subcutaneous "backpack" tumors in syngeneic mice, resulting in secretion of monoclonal sIgA onto mucosal surfaces. Neonatal mice bearing anti-lipopolysaccharide hybridoma backpack tumors were specifically protected against oral challenge with 100 50% lethal doses of virulent Ogawa 395 organisms. Thus, the IgA hybridoma backpack tumor method identifies protective epitopes in the mucosal system and demonstrates that a single monoclonal sIgA can be sufficient to protect against intestinal disease.
机译：分泌型免疫球蛋白A（sIgA）在抵抗霍乱弧菌和其他感染粘膜表面的微生物中起着一定的作用，但尚不确定单独使用sIgA是否可以预防疾病。我们在这里报告了一种鉴定能够提供这种保护的单克隆sIgA抗体的抗原特异性的策略。用霍乱弧菌小川395口服免疫后，从派伊尔氏淋巴结淋巴细胞产生IgA杂交瘤。选择一个克隆，该克隆产生针对暴露于细菌表面的小川特异性脂多糖碳水化合物抗原的二聚体单克隆IgA抗体。杂交瘤细胞用于在同系小鼠中产生皮下“背包”肿瘤，导致单克隆sIgA分泌到粘膜表面。携带抗脂多糖杂交瘤背包肿瘤的新生小鼠受到了100％50％致死剂量的强效Ogawa 395生物体的口服攻击的特别保护。因此，IgA杂交瘤背包肿瘤方法可识别粘膜系统中的保护性抗原决定簇，并证明单个单克隆sIgA足以预防肠道疾病。

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