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首页> 外文期刊>Infection and immunity >Adherence of oral 'Streptococcus milleri' cells to surfaces in broth cultures.
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Adherence of oral 'Streptococcus milleri' cells to surfaces in broth cultures.

机译：口服“链球菌”细胞粘附于肉汤培养物中的表面。

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Cells of representative strains of oral "Streptococcus milleri" firmly adhered to glass surfaces when grown in glucose broths as well as in fructose and sucrose broths. Cellular adherence occurred on saliva-coated glass surfaces as well as uncoated surfaces, but the cells of only a few (6 of 69) tested adherent strains agglutinated upon the addition of human whole-saliva supernatant. Thus, the firm adherence of growing "S. milleri" cells is independent of sucrose and salivary macromolecules. Two macroscopic forms of cell adherence were observed: one was a coarse-granule type and one was a tiny-granule type covered with a thin homogeneous membranous material. Scanning electron microscopy revealed that, in both types, a stringy substance interconnected the cells and connected the cells to glass surfaces, with amorphous flocklike materials present in the intercellular space. Of the 154 tested strains, 128 were adherent, of which 90 were firmly and extensively (+2 or +3) adherent. Most strains of the serovars f/F, g, h, i, j, g-, and gj- were extensively adherent. Generally, cells of the more strongly adherent strains were more hydrophobic and spontaneously aggregating. However, there also existed a few hydrophilic and nonaggregating but extensively adherent strains. The presence of trypsin or pronase completely inhibited the adherence of some strains (mostly nonaggregating) but did not at all inhibit that of others (mostly aggregating). The adhering groups did not significantly differ in their cell surface hydrophobicities. Therefore, at least two factors, one proteinaceous and one nonproteinaceous, appear to be principally involved in the firm surface adherence of "S. milleri."
机译：当在葡萄糖肉汤以及果糖和蔗糖肉汤中生长时，口服“ Streptococcus milleri”代表性菌株的细胞牢固地粘附在玻璃表面上。细胞粘附发生在唾液包被的玻璃表面和未涂布的表面上，但是只有少数（69个中的6个）被测试的粘附菌株的细胞在添加人全唾液上清液后凝集。因此，生长的“ S.milleri”细胞的牢固粘附独立于蔗糖和唾液大分子。观察到两种细胞粘附的宏观形式：一种是粗颗粒型，一种是细颗粒型，上面覆盖着一层薄薄的均质膜材料。扫描电子显微镜显示，在两种类型中，一种线状物质将细胞相互连接并将细胞连接至玻璃表面，且细胞间空间内存在无定形的绒毛状材料。在154个测试菌株中，有128个是粘附的，其中90个是牢固和广泛（+2或+3）粘附的。血清型f / F，g，h，i，j，g-和gj-的大多数菌株都广泛附着。通常，粘附力更强的菌株的细胞更疏水并且自发聚集。但是，也存在一些亲水性和非聚集性但广泛粘附的菌株。胰蛋白酶或链霉蛋白酶的存在完全抑制了某些菌株的粘附（主要是非聚集的），但完全没有抑制其他菌株的粘附（主要是聚集的）。粘附基团的细胞表面疏水性没有显着差异。因此，至少两个因素，一种蛋白质和一种非蛋白质，似乎主要参与了“ S. milleri”的牢固表面粘附。

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