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首页> 外文期刊>Infection and immunity >Persistence of Escherichia coli bacteriuria is not determined by bacterial adherence.
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Persistence of Escherichia coli bacteriuria is not determined by bacterial adherence.

机译：大肠埃希氏菌的持久性不是由细菌的依附性决定的。

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The role of bacterial adherence in the persistence of bacteria in the human urinary tract was analyzed. Women with chronic symptomatic urinary tract infections were subjected to deliberate colonization with nonvirulent Escherichia coli, after eradication of their current infections. E. coli organisms were instilled into the bladder through a catheter. The strain used for colonization, E. coli 83972, was isolated from a patient with stable bacteriuria. It lacked expressed adherence factors and did not belong to the uropathogenic O:K:H serotypes. Strain 83972 was transformed with the pap and pil DNA sequences encoding Gal alpha 1-4Gal beta- and mannose-specific (type 1) adhesins. Patients were colonized with a mixture of the wild-type and the transformed strains. E. coli 83972 caused stable bacteriuria for greater than 30 days in 7 of 12 individuals. In contrast, the Gal alpha 1-4 Gal beta-recognizing or mannose-binding transformants were eliminated within 48 h. The consistent superiority of the wild-type strain in establishing stable bacteriuria when compared with the adhesive transformants did not appear to be due to differences in growth rates or to plasmid segregation. Rather, the transformants expressing the adhesin determinants were selectively eliminated by the host. This suggested that the acquisition of adherence factors is not sufficient to increase the fitness of E. coli for survival in the urinary tract of humans.
机译：分析了细菌粘附在人尿道中细菌持久性中的作用。根除当前感染后，患有慢性症状性尿路感染的妇女必须接受无毒大肠杆菌的故意定植。通过导管将大肠杆菌生物体滴入膀胱。从具有稳定细菌尿症的患者中分离出用于定殖的菌株大肠杆菌83972。它缺乏明确的依从性因子，不属于尿毒症性O：K：H血清型。用编码Gal alpha 1-4Galβ-和甘露糖特异性（1型）粘附素的pap和pil DNA序列转化菌株83972。患者用野生型和转化菌株的混合物定殖。大肠杆菌83972在12个人中的7个人中引起稳定的菌尿超过30天。相反，在48小时内消除了识别Gal Gal 1-4 Gal Gal或甘露糖结合的转化子。当与粘附转化体相比时，野生型菌株在建立稳定的细菌尿中的一致优势似乎不是由于生长速率的差异或质粒的分离。而是，宿主选择性地消除了表达粘附素决定簇的转化体。这表明获得粘附因子不足以增加大肠杆菌在人尿道中存活的适应性。

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