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首页> 外文期刊>Infection and immunity >Interaction of the fish pathogen Aeromonas salmonicida with rainbow trout macrophages.
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Interaction of the fish pathogen Aeromonas salmonicida with rainbow trout macrophages.

机译：鱼病原体鲑鱼气单胞菌与虹鳟巨噬细胞的相互作用。

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A procedure was developed to culture rainbow trout macrophages (M phi) on supported glass coverslips. Using this method and a variety of well-characterized Aeromonas salmonicida strains with normal or altered cell surfaces, we investigated the role of this unusual bacterial surface in the bacterium-M phi interaction. An intact crystalline protein array, the A-layer, mediated adherence of A. salmonicida cells to M phi even in the absence of opsonins. In contrast, unopsonized cells of an A-layer-negative (A-) mutant with a smooth lipopolysaccharide (LPS) layer were unable to interact with M phi. However, this ability was recovered when the A-layer was reconstituted onto the smooth LPS surface of these A- LPS+ cells. Two A. salmonicida mutants possessing the A-layer in different disorganized states had a reduced ability to interact with M phi. A+ cells grown under calcium limitation produced A-layers locked into an alternative conformation which mediated the highest levels of M phi association in the absence of opsonins or any other surface coating. Coating A+ cells with hemin greatly increased their levels of M phi association, and bacterial cells grown on trout blood agar plates also had a dramatic increase in their ability to interact with M phi. Only A+ A. salmonicida cells were highly cytotoxic to trout M phi, especially after being coated with hemin, presumably due to a more focused targeting of the bacterial cell onto the M phi surface and/or into the intracellular regions of the M phi.
机译：开发了在支撑的玻璃盖玻片上培养虹鳟巨噬细胞（M phi）的程序。使用此方法和具有正常的或更改的细胞表面的各种特性良好的鲑鱼气单胞菌菌株，我们调查了这种异常细菌表面在细菌-M phi相互作用中的作用。一个完整的晶体蛋白阵列，即A层，即使在没有调理素的情况下，也能介导鲑鱼曲霉细胞对M phi的粘附。相反，具有平滑脂多糖（LPS）层的A层阴性（A-）突变体的未调理细胞不能与M phi相互作用。然而，当将A层重构到这些A-LPS +细胞的光滑LPS表面上时，恢复了这种能力。具有不同杂乱状态的A层的两个沙门氏菌突变体与M phi相互作用的能力降低。在钙限制下生长的A +细胞产生的A层被锁定为另一种构象，在没有调理素或任何其他表面涂层的情况下，介导了最高水平的M phi缔合。用血红素包被A +细胞大大提高了其M phi结合水平，并且在鳟鱼血琼脂平板上生长的细菌细胞与M phi相互作用的能力也有了显着提高。仅沙门氏菌（A +）沙门氏菌细胞对鳟鱼M phi具有很高的细胞毒性，尤其是在被血红素包被之后，这可能是由于细菌细胞更集中地靶向M phi表面和/或M phi的细胞内区域。

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