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首页> 外文期刊>Infection and immunity >Lipopolysaccharide (LPS) from Brucella abortus is less toxic than that from Escherichia coli, suggesting the possible use of B. abortus or LPS from B. abortus as a carrier in vaccines.
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Lipopolysaccharide (LPS) from Brucella abortus is less toxic than that from Escherichia coli, suggesting the possible use of B. abortus or LPS from B. abortus as a carrier in vaccines.

机译：流产布鲁氏菌的脂多糖（LPS）的毒性比大肠杆菌低，表明可能将流产布鲁氏菌或流产布鲁氏菌的LPS用作疫苗的载体。

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Brucella abortus may be useful as a component of vaccines. This is because it possesses several unique properties as a carrier that enable it to stimulate human B cells even in the relative absence of T cells. Human immunodeficiency virus type 1 proteins conjugated to B. abortus could induce neutralizing antibodies against human immunodeficiency virus type 1. Recently we showed that the characteristics of lipopolysaccharide (LPS) derived from B. abortus are similar to those of the whole bacterium in that the LPS acts as a T-independent type 1 carrier in mice. In this study we wanted to determine whether LPS derived from B. abortus is associated with the adverse effects seen with other bacterial endotoxins. LPS purified from B. abortus by butanol extraction was shown to have less than 2% (wt/wt) contamination by protein and less than 1% (wt/wt) contamination by nucleic acids and to contain 1% (wt/wt) ketodeoxyoctanic acid. Compared with LPS derived from Escherichia coli, B. abortus LPS was 10,000-fold less potent in eliciting fever in rabbits, 268-fold less potent in killing D-galactosamine-sensitized mice, and 1,400-fold and 400-fold less potent in inducing interleukin-1 beta and tumor necrosis factor alpha production, respectively. These results suggest that B. abortus LPS is much less likely than the LPS from E. coli to evoke endotoxic shock; therefore, it may be feasible to incorporate B. abortus as a component of vaccines.
机译：流产布鲁氏菌可用作疫苗的成分。这是因为它具有几种独特的载体性质，即使在相对缺乏T细胞的情况下也能刺激人B细胞。与流产双歧杆菌结合的人免疫缺陷病毒1型蛋白可诱导针对人免疫缺陷病毒1型的中和抗体。最近，我们发现源自流产芽孢杆菌的脂多糖（LPS）的特征与整个细菌的相似之处在于，LPS在小鼠中充当T非依赖性1型载体。在这项研究中，我们想确定源自流产双歧杆菌的LPS是否与其他细菌内毒素所见的不良反应有关。通过丁醇提取从流产双歧杆菌中纯化的LPS被蛋白质污染小于2％（wt / wt），而核酸污染小于1％（wt / wt），并且含有1％（wt / wt）酮氧基辛酸酸。与衍生自大肠杆菌的LPS相比，流产芽孢杆菌LPS引起兔子发烧的效力低10,000倍，杀死D-半乳糖胺致敏小鼠的效力低268倍，诱导诱导效力低1,400倍和400倍白细胞介素-1β和肿瘤坏死因子α的产生。这些结果表明，流产芽孢杆菌LPS引起大肠杆菌内毒素休克的可能性远低于大肠杆菌的LPS。因此，将流产双歧杆菌作为疫苗的组成部分是可行的。

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