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首页> 外文期刊>Infection and immunity >Effect of pH and osmolality on in vitro phagocytosis and killing by neutrophils in urine.
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Effect of pH and osmolality on in vitro phagocytosis and killing by neutrophils in urine.

机译：pH和渗透压对尿液中性粒细胞体外吞噬作用和杀伤力的影响。

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Phagocytosis and intracellular killing of two strains of Escherichia coli and a Staphylococcus saprophyticus by polymorphonuclear neutrophils (PMN) in pooled sterile urine at three osmolalities (800, 485, and 200 mosM/kg of H2O) between pHs 5 and 8 was investigated. Urine at 800 mosM virtually abolished phagocytosis of both E. coli strains, regardless of pH, and reduced the phagocytosis of S. saprophyticus to 30%; no killing of any organisms took place at this osmolality. On the other hand, phagocytosis was a good in urine as in Hanks balanced salt solution at both 485 and 200 mosM between pHs 6 and 8. Phagocytosis of all three strains was virtually abolished at pH 5. Killing of the strains by PMN was optimal between pHs 6.5 and 7.5 in urine at 485 mosM (being at least 90% of the control values in Hanks balanced salt solution), whereas at 200 mosM killing was reduced to 50 to 70% of these values. Reduced killing of all three strains occurred at pH 8, whereas at pH 6 only S. saprophyticus was killed. Thus, the bactericidal activity of PMN in urine was more sensitive than phagocytic function to alterations in pH. The dominant modulating factor affecting PMN function in urine of 500 mosM or less was pH, but osmolality had a greater influence at 800 mosM. Thus, raising the pH of urine and reducing the osmolality may increase the ability of natural defense mechanisms to eliminate infecting organisms.
机译：研究了多形核中性粒细胞（PMN）在pH 5和8之间的三个渗透压（800、485和200 mosM / kg H2O）汇集的无菌尿液中吞噬多形核中性粒细胞（PMN）对两株大肠杆菌和腐生葡萄球菌的吞噬作用和细胞内杀伤作用。 800 mosM的尿液实际上消除了两种大肠杆菌菌株的吞噬作用，无论其pH值如何，都将腐生链球菌的吞噬作用降低至30％。在该重量克分子渗透浓度下，没有杀死任何生物。另一方面，在pH值为6和8的485和200 mosM之间，在Hanks平衡盐溶液中，吞噬作用很好。在pH 5时，所有这三株菌株的吞噬作用基本都消失了。 485 mosM时尿液中的pH值6.5和7.5（至少是汉克斯平衡盐溶液中对照值的90％），而200 mosM时，杀死率降至这些值的50％至70％。在pH 8时，所有三种菌株的杀灭力均降低，而在pH 6时，只有腐生链球菌被杀死。因此，尿液中PMN的杀菌活性比吞噬功能对pH的变化更敏感。 500 mosM以下的尿液中影响PMN功能的主要调节因子是pH，但渗透压在800 mosM时影响更大。因此，提高尿液的pH值和降低重量克分子渗透压浓度可以提高自然防御机制消除感染生物的能力。

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