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首页> 外文期刊>Infection and immunity >Protective immunity in murine histoplasmosis: functional comparison of adoptively transferred T-cell clones and splenic T cells.
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Protective immunity in murine histoplasmosis: functional comparison of adoptively transferred T-cell clones and splenic T cells.

机译：小鼠组织胞浆菌病的保护性免疫：过继转移的T细胞克隆和脾T细胞的功能比较。

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In this study, I examined whether a murine T-cell line and three clones that recognize Histoplasma capsulatum antigens in vitro could confer protection in vivo against a challenge of Histoplasma yeasts. C57BL/6 mice were each inoculated with 5 X 10(4) yeasts intravenously; 1 h later, 5 X 10(6) or 2 X 10(7) resting T cells were inoculated intravenously. At week 1 of infection, the T-cell line and all clones failed to reduce the number of H. capsulatum CFU in the spleens of mice compared with numbers in infected controls. Administration of recombinant interleukin 2 or cyclophosphamide to infected mice did not potentiate the functional activity in vivo of either the T-cell line or the clones. In contrast, inoculation with 2 X 10(7) CD4+ but not CD8+ cells isolated from the spleens of mice immunized with 10(6) viable yeast cells sharply diminished the number of CFU in the spleens of infected animals. Moreover, splenic CD4+ cells from immune mice transferred a delayed-type hypersensitivity response, whereas the T-cell line and clones did not. Injection Injection of an equal number of cloned T cells and CD8+ splenocytes from immune mice did not transfer resistance to infected mice. Additional studies were undertaken to determine if the ineffectiveness of cloned T cells was associated with a failure to migrate to and survive within spleens of infected mice. B6.PL Thy-1a/Cy mice, which are genetically identical to C57BL/6 mice except that T cells of the former bear Thy-1.1 rather than Thy-1.2, were inoculated with Histoplasma yeasts and then injected with immune CD4+ splenocytes or a T-cell clone. At days 1 and 7 of infection, virtually no Thy-1.2+ cells were detected in the spleens of infected mice given cloned T cells. However, the spleens of animals inoculated with immune CD4+ cells contained a small but significant (P less than 0.01) proportion of Thy-1.2+ cells at both day 1 and day 7 postinoculation of H. capsulatum. Thus, the failure of T-cell clones to transfer protection against H. capsulatum may be explained by defective trafficking or poor survival in vivo or both.
机译：在这项研究中，我检查了鼠类T细胞系和三个在体外识别荚膜胞浆抗原的克隆是否可以在体内提供保护，以抵御挑战。分别给C57BL / 6小鼠静脉内接种5 X 10（4）个酵母菌。 1小时后，静脉内接种5 X 10（6）或2 X 10（7）静息T细胞。与感染的对照组相比，在感染的第1周，T细胞系和所有克隆均未能减少小鼠脾脏中荚膜幽门螺杆菌CFU的数量。向感染的小鼠施用重组白介素2或环磷酰胺不能增强T细胞系或克隆的体内功能活性。相比之下，接种2 X 10（7）CD4 +细胞而不接种CD8 +细胞，这些细胞是从用10（6）有活力酵母细胞免疫的小鼠的脾脏中分离的，大大减少了被感染动物脾脏中CFU的数量。此外，来自免疫小鼠的脾脏CD4 +细胞转移了迟发型超敏反应，而T细胞系和克隆则没有。注射从免疫小鼠中注射相同数量的克隆T细胞和CD8 +脾细胞不会将抗性转移给感染的小鼠。进行了进一步的研究，以确定克隆的T细胞的无效性是否与迁移到受感染小鼠的脾脏和在脾脏中生存有关。 B6.PL Thy-1a / Cy小鼠与C57BL / 6小鼠在基因上相同，除了前者的T细胞带有Thy-1.1而不是Thy-1.2，然后接种了组织胞浆酵母，然后注射了免疫CD4 +脾细胞或T细胞克隆。在感染的第1天和第7天，在给予克隆T细胞的感染小鼠脾脏中几乎未检测到Thy-1.2 +细胞。然而，在接种荚膜梭状芽胞杆菌后第1天和第7天，接种免疫CD4 +细胞的动物的脾脏中含有少量但显着（P小于0.01）的Thy-1.2 +细胞。因此，T细胞克隆不能转移针对荚膜梭状芽胞杆菌的保护的失败可能是由于有缺陷的运输或体内不良的存活或两者兼而有之。

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