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首页> 外文期刊>Infection and immunity >Characterization of a 78-kilodalton outer membrane protein of Haemophilus somnus.
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Characterization of a 78-kilodalton outer membrane protein of Haemophilus somnus.

机译：嗜血嗜血杆菌的78千达尔顿外膜蛋白的表征。

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A 78-kilodalton (kDa) outer membrane protein (OMP) of Haemophilus somnus was one of the two antigens most consistently and most intensely immunoreactive in Western immunoblots of whole cells of H. somnus reacted with convalescent-phase serum obtained from cattle with experimental H. somnus pneumonia. This antigen was isolated by gel filtration chromatography of sodium dodecyl sulfate-solubilized OMP. Reactions of Western blots with bovine monospecific antiserum prepared against the 78-kDa antigen indicated that this 78-kDa OMP was present in each of 22 isolates of H. somnus obtained from cattle with pneumonia, thromboembolic meningoencephalitis, and abortion as well as from vaginal or preputial carriers. The 78-kDa OMP was also present in each isolate obtained weekly throughout the course of experimental H. somnus pneumonia in a calf. Monospecific antiserum to the 78-kDa OMP also reacted with proteins from closely related bacterial species in the family Pasteurellaceae but not with bacteria of 13 other genera. The 78-kDa OMP of H. somnus is of interest because it is surface accessible, highly conserved, immunogenic, cross-reactive with other members of the family Pasteurellaceae, and reactive with convalescent-phase serum which is passively protective against H. somnus pneumonia.
机译：嗜血嗜血杆菌的78千达尔顿（kDa）外膜蛋白（OMP）是在嗜血链球菌全细胞的Western免疫印迹中最一致和最强免疫反应性的两种抗原之一，与从实验H牛获得的恢复期血清反应嗜睡性肺炎通过十二烷基硫酸钠溶解的OMP的凝胶过滤色谱法分离该抗原。 Western印迹与针对78 kDa抗原制备的牛单特异性抗血清的反应表明，从患有肺炎，血栓栓塞性脑膜脑炎和流产的牛，流产以及从阴道或阴道中获得的22株猪嗜血杆菌中均存在该78 kDa OMP。特级载体。在小牛的整个实验性猪链球菌肺炎的过程中，每周获得的每个分离物中也存在78 kDa OMP。 78 kDa OMP的单特异性抗血清也与巴斯德氏菌科中密切相关的细菌物种的蛋白质反应，但不与其他13个属的细菌反应。猪链球菌的78 kDa OMP是令人感兴趣的，因为它表面可接触，高度保守，具有免疫原性，可与巴斯德氏菌科的其他成员发生交叉反应，并且与恢复期血清具有反应性，而后者对猪链球菌肺炎具有被动保护作用。 。

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