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首页> 外文期刊>Infection and immunity >Restoration of exocytosis occurs after inactivation of intracellular tetanus toxin.
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Restoration of exocytosis occurs after inactivation of intracellular tetanus toxin.

机译：胞内破伤风毒素灭活后发生胞吐作用的恢复。

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Tetanus toxin blocks carbachol-stimulated release of noradrenaline from bovine adrenal chromaffin cells in culture, provided it can gain access to the cells. This can be achieved by electropermeabilization of the plasma membrane or by enriching the membrane with exogenous gangliosides which serve as carriers of the toxin. The inhibition of noradrenaline release persists for at least 6 days, even in the presence of specific anti-tetanus toxin antibodies in the culture medium. However, the block is preventable, for the most part, when antibodies enter chromaffin cells during electropermeabilization, before the uptake of the toxin is facilitated by inserting exogenous gangliosides into the plasma membrane 2 days later. This indicates that the antibodies pass into the cells through the physically induced pores and that these intracellular antibodies neutralize incoming tetanus toxin. If, on the other hand, exocytosis has been inhibited by tetanus toxin, it will recover within 3 days, provided specific anti-tetanus toxin antibodies are introduced into the cells by electropermeabilization. The recovery is not linked to a specific route of entry of the toxin. It is concluded that the restoration of noradrenaline release requires not only the intracellular neutralization of tetanus toxin but also the reconstitution of the as yet unknown target molecule of the toxin.
机译：破伤风毒素可以阻断培养物中牛肾上腺嗜铬细胞中卡巴胆碱刺激的去甲肾上腺素的释放，只要它能进入细胞即可。这可以通过质膜的电透化或通过用作为毒素载体的外源神经节苷脂富集膜来实现。即使在培养基中存在特异性抗破伤风毒素抗体的情况下，去甲肾上腺素释放的抑制也持续至少6天。但是，在大多数情况下，当抗体在电透化过程中进入嗜铬细胞时，在2天后将外源神经节苷脂插入质膜促进毒素吸收之前，这种阻断作用是可以预防的。这表明抗体通过物理诱导的孔进入细胞，并且这些细胞内抗体中和了传入的破伤风毒素。另一方面，如果破伤风毒素抑制了胞吐作用，只要通过电通透性将特定的抗破伤风毒素抗体引入细胞，它就会在3天内恢复。恢复与毒素的特定进入途径无关。结论是，去甲肾上腺素释放的恢复不仅需要破伤风毒素的细胞内中和作用，而且还需要重建毒素的未知靶分子。

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