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首页> 外文期刊>Infection and immunity >Variation in the structure of glucuronoxylomannan in isolates from patients with recurrent cryptococcal meningitis.
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Variation in the structure of glucuronoxylomannan in isolates from patients with recurrent cryptococcal meningitis.

机译：复发性隐球菌性脑膜炎患者分离物中葡糖醛酸氧甘露聚糖的结构变化。

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Capsular glucuronoxylomannans (GXM) of Cryptococcus neoformans var. neoformans isolates from patients with recurrent cryptococcal meningitis were analyzed by 1H nuclear magnetic resonance spectroscopy and for reactivity with factor sera (Iatron, Tokyo, Japan). For each patient the initial and relapse isolates had previously been shown to be indistinguishable by DNA restriction fragment length polymorphism analysis. For patients J11 and J22 the GXM of the initial and relapse isolates were identical. For patients SB4 and SB6 the GXM of the initial and relapse isolates differed in structure and reactivity with factor sera. In patient SB4 the initial isolate had a serotype A/D structure, and the first relapse isolate had a serotype A structure. The second relapse isolate was a mixture of structures composed of serotype D components, glucuronomannan (GM), and a minor serotype A component. Analysis of the initial isolate from patient SB6 showed a structure composed mainly of serotype D, GM, and minor serotype A components and components not assigned to a particular serotype (N). The relapse isolate had the same composition as the initial isolate except for an increase in the serotype A component. This increase in the serotype A component of the relapse isolate resulted in a change in the serological specificity from serotype D to serotype A/D. The initial isolate from patient J9 had serotype D and GM structures. The first two relapse isolates had serotype D, N, and GM structures and a minor serotype A component. The third relapse isolate had mainly a serotype D structure. All the J9 isolates reacted only with serotype D-specific factor serum. These results indicate that some isolates obtained from patients with recurrent C. neoformans infections have undergone a change in GXM structure during the course of infection. The modification of GXM structure observed in some relapse isolates is reflected in changed serological properties. The results may have important implications for the design of vaccines and antibody-based therapeutic strategies against C. neoformans.
机译：新型隐球菌变种的荚膜葡糖醛酸羟甘露聚糖（GXM）。通过1H核磁共振波谱分析了复发性隐球菌性脑膜炎患者的新分离株，并分析了其与因子血清的反应性（Iatron，东京，日本）。对于每位患者，先前通过DNA限制性片段长度多态性分析无法区分出最初和复发的分离株。对于患者J11和J22，初始分离株和复发分离株的GXM相同。对于SB4和SB6患者，初始和复发分离株的GXM在结构和与因子血清的反应性方面有所不同。在SB4患者中，最初的分离株具有血清型A / D结构，而第一个复发分离株具有A型血清型结构。第二个复发分离株是由D型血清成分，葡萄糖醛酸甘露聚糖（GM）和A型次要血清成分组成的结构的混合物。对来自患者SB6的初始分离物的分析表明，该结构主要由D型，GM型和次要A型血清成分以及未分配给特定血清型（N）的成分组成。复发分离株具有与初始分离株相同的组成，除了血清型A组分增加。复发分离株的血清型A组分的这种增加导致血清特异性从血清型D改变为血清型A / D。来自患者J9的最初分离株具有血清型D和GM结构。前两个复发分离株具有血清型D，N和GM结构以及次要的血清型A成分。第三复发分离株主要具有血清型D结构。所有J9分离株仅与血清型D特异性因子血清反应。这些结果表明，从复发性新孢子虫感染患者获得的一些分离株在感染过程中发生了GXM结构的变化。在一些复发分离株中观察到的GXM结构的改变反映在改变的血清学性质中。该结果可能对疫苗的设计和基于抗体的抗新孢子虫的治疗策略具有重要意义。

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