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首页> 外文期刊>Infection and immunity >Effects of the two varieties of Cryptococcus neoformans cells and culture filtrate antigens on neutrophil locomotion.
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Effects of the two varieties of Cryptococcus neoformans cells and culture filtrate antigens on neutrophil locomotion.

机译：两种新隐球菌细胞和培养滤液抗原对嗜中性粒细胞运动的影响。

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Cryptococcus neoformans var. gattii (serotype B and C) isolates have a relative predilection for immunocompetent hosts, and C. neoformans var. neoformans (serotype A and D) isolates have a relative predilection for immunocompromised hosts, suggesting that normal host resistance to the former may be relatively inefficient compared with that to the latter variety. In order to assess the possibility that normal cellular host defense is inadequate in protecting against C. neoformans var. gattii, we compared the two varieties of C. neoformans cells and their culture filtrate antigens (CneF) with respect to effects on neutrophil (polymorphonuclear leukocyte [PMN]) locomotion. In a 48-well modified Boyden chamber, the cells and CneF of C. neoformans var. neoformans (serotype A and D) isolates stimulated chemotaxis and chemokinesis of human PMN and activated a complement component(s) in pooled human serum to become a chemoattractant(s) for human PMN. In contrast, the cells and CneF of C. neoformans var. gattii (serotype B and C) isolates did not stimulate chemotaxis or chemokinesis in human PMN but rather inhibited chemokinesis and chemotactic responses of PMN to pooled human serum and formylmethionyl leucyl phenylalanine. Neither of the CneF from the C. neoformans var. gattii isolates was cytotoxic to PMN. Furthermore, with the mouse model, we found that CneF from C. neoformans var. neoformans caused migration of PMN into gelatin sponges implanted in naive and immunized mice, whereas CneF from C. neoformans var. gattii inhibited PMN migration into sponges. Our results, combined with findings of others showing reduced PMN infiltration in lungs of mice infected with C. neoformans var. gattii compared with PMN infiltration in lungs of mice infected with C. neoformans var. neoformans, indicate that the relative inadequacy of normal host resistance mechanisms to prevent infection with C. neoformans var. gattii results, in part, from inhibition of PMN migration to the site of the organism.
机译：新型隐球菌变种加蒂（B型和C型血清型）分离株对具有免疫能力的宿主和新孢梭菌变种具有相对优势。新甲虫（血清型A和D）的分离株对免疫功能低下的寄主具有相对的优势，这表明与前者相比，对前者的正常寄主抗性可能效率相对较低。为了评估正常的细胞宿主防御能力不足以预防新孢子虫变种的可能性。加蒂，我们就嗜中性白血球（多形核白细胞[PMN]）运动的影响比较了两种新形成的梭状芽胞杆菌细胞及其培养滤液抗原（CneF）。在一个48孔改良的博伊登室中，新孢梭菌的细胞和CneF变种。新甲虫（血清型A和D）分离出刺激的人类PMN趋化性和趋化因子，并激活了人血清中的补体成分，成为人类PMN的趋化剂。相反，新孢梭菌的细胞和CneF。加蒂（B型和C型）分离物不会刺激人PMN的趋化性或趋化性，但会抑制PMN对合并的人血清和甲酰甲硫基亮氨酰苯丙氨酸的趋化性和趋化性反应。新孢梭菌的两个CneF都没有。加蒂分离株对PMN具有细胞毒性。此外，在小鼠模型中，我们发现来自新孢梭菌的CneF。 Neoformans导致PMN迁移到植入天真和免疫小鼠的明胶海绵中，而来自C. Neoformans的CneF变种。加蒂抑制了PMN迁移到海绵中。我们的研究结果与其他研究结果相结合，显示新感染梭状芽胞杆菌变种的小鼠肺内PMN浸润减少。加蒂病毒与PMN浸润感染新孢子虫变种小鼠的肺相比。 Neoformans，表明正常的宿主抗药性机制相对不足，无法预防C. neoformans var的感染。加蒂的部分原因是抑制了PMN迁移到生物体的部位。

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