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首页> 外文期刊>Infection and immunity >Interleukin-12 Production Is Required for Chlamydial Antigen-Pulsed Dendritic Cells To Induce Protection against Live Chlamydia trachomatis Infection
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Interleukin-12 Production Is Required for Chlamydial Antigen-Pulsed Dendritic Cells To Induce Protection against Live Chlamydia trachomatis Infection

机译：衣原体抗原脉冲树突状细胞需要白细胞介素12的生产，以诱导对沙眼衣原体感染的保护。

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Immunization with dendritic cells pulsed ex vivo with antigens has been successfully used to elicit primary antigen-specific immune responses. We report that mouse bone marrow-derived dendritic cells pulsed with inactivated chlamydial organisms induced strong protection against live chlamydial infection in a mouse lung infection model. Either the dendritic cells or chlamydial organisms alone or macrophages similarly pulsed with chlamydial organisms failed to induce any significant protection. These observations suggest that dendritic cells can efficiently process and present chlamydial antigens to naive T cells in vivo. Mice immunized with the chlamydia-pulsed dendritic cells preferentially developed a Th1 cell-dominant response while mice immunized with the other immunogens did not, suggesting a correlation between a Th1 cell-dominant response and protection against chlamydial infection. We further found that dendritic cells produced a large amount of interleukin 12 (IL-12) upon ex vivo pulsing with inactivated chlamydial organisms, which may allow the dendritic cells to direct a Th1 cell-dominant response. Dendritic cells from mice deficient in the IL-12 p40 gene failed to produce IL-12 after a similar ex vivo pulse with chlamydial organisms, and more importantly, immunization with these dendritic cells failed to induce a Th1 cell-dominant response and did not induce strong protection against chlamydial infection. Thus, the ability of dendritic cells to efficiently process and present chlamydial antigens and to produce IL-12 upon chlamydial-organism stimulation are both required for the induction of protection against chlamydial infection. This information may be useful for the further design of effective chlamydial vaccines.
机译：用抗原离体脉冲的树突状细胞进行的免疫已成功用于引发主要抗原特异性免疫反应。我们报告说灭活的衣原体微生物脉冲小鼠骨髓来源的树突状细胞在小鼠肺部感染模型中诱导针对活衣原体感染的强大保护。单独的树突状细胞或衣原体生物或类似地用衣原体生物脉冲的巨噬细胞均不能诱导任何显着的保护作用。这些观察结果表明，树突状细胞可以在体内有效地处理衣原体抗原并将其呈递给幼稚T细胞。用衣原体脉冲树突状细胞免疫的小鼠优先产生以Th1细胞为主的应答，而用其他免疫原免疫的小鼠则没有，这表明Th1细胞以应答与针对衣原体感染的保护之间存在相关性。我们进一步发现树突状细胞在用灭活的衣原体生物体进行离体脉冲后产生大量白介素12（IL-12），这可能使树突状细胞指导Th1细胞主导的反应。与衣原体生物类似的离体脉冲后，来自缺乏IL-12 p40基因的小鼠的树突状细胞未能产生IL-12，更重要的是，用这些树突状细胞进行的免疫未能诱导Th1细胞主导的反应，并且没有诱导对衣原体感染的有力保护。因此，树突状细胞有效地加工和呈递衣原体抗原的能力以及在衣原体-生物刺激时产生IL-12的能力都是诱导针对衣原体感染的保护所必需的。此信息可能对进一步设计有效的衣原体疫苗有用。

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