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Roles of the Surface Layer Proteins ofCampylobacter fetus subsp. fetus in Ovine Abortion

机译：弯曲杆菌胎儿亚种表面层蛋白的作用胎儿在​​堕胎中

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The role of the surface (S)-layer proteins of Campylobacter fetus subsp. fetus has been investigated using an ovine model of abortion. Wild-type strain 23D induced abortion in up to 90% of pregnant ewes challenged subcutaneously. Isolates recovered from both dams and fetuses expressed S-layer proteins with variable molecular masses. The spontaneous S-layer-negative variant, strain 23B, neither colonized nor caused abortions in pregnant ewes. A series of isogenic sapA and recA mutants, derived from 23D, also were investigated in this model. A mutant (501 [sapA recA +]) caused abortion in one of five challenged animals and was recovered from the placenta of a second animal. Another mutant (502 [sapA recA]) with no S-layer protein expression caused no colonization or abortions in challenged animals but caused abortion when administered intraplacentally. Mutants 600(2) and 600(4), both recA, had fixed expression of 97- and 127-kDa S-layer proteins, respectively. Two of the six animals challenged with mutant 600(4) were colonized, but there were no abortions. As expected, all five strains recovered expressed a 127-kDa S-layer protein. In contrast, mutant 600(2) was recovered from the placentas of all five challenged animals and caused abortion in two. Unexpectedly, one of the 16 isolates expressed a 127-kDa rather than a 97-kDa S-layer protein. Thus, these studies indicate that S-layer proteins appear essential for colonization and/or translocation to the placenta but are not required to mediate fetal injury and that S-layer variation may occur in a recA strain.
机译：弯曲杆菌胎儿亚种表面（S）层蛋白的作用。已经使用绵羊流产模型对胎儿进行了研究。野生型23D菌株可在高达90％的皮下受孕母羊中流产。从大坝和胎儿中回收的分离物均表达了分子量可变的S层蛋白。自发的S层阴性变异株23B，在怀孕的母羊中既不定植也不引起流产。在此模型中还研究了衍生自23D的一系列同基因sapA和recA突变体。突变体（501 [sapA recA +]）导致五只受攻击动物之一流产，并从第二只动物的胎盘中回收。没有S层蛋白表达的另一种突变体（502 [sapA recA]）在受攻击的动物中没有引起定植或流产，但是当通过胎盘内给药时却引起了流产。突变体600（2）和600（4）均为recA，分别具有97-kDa和127-kDa S层蛋白的固定表达。用突变体600（4）攻击的六只动物中有两只被定居，但没有流产。如预期的那样，回收的所有五个菌株均表达了127 kDa S层蛋白。相比之下，突变体600（2）从所有五只受攻击动物的胎盘中回收，并导致其中两人流产。出乎意料的是，这16个分离株之一表达的是127 kDa而不是97 kDa的S层蛋白。因此，这些研究表明，S层蛋白似乎对于定植和/或易位至胎盘必不可少，但不需要介导胎儿损伤，并且在recA菌株中可能会发生S层变异。

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