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The Global Regulator ArcA Modulates Expression of Virulence Factors in Vibrio cholerae

机译：全球监管机构ArcA调节霍乱弧菌中毒力因子的表达。

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A Vibrio cholerae arcA mutant was constructed and used to examine the role of the global anaerobiosis response regulator ArcA in the expression of virulence factors in this important human pathogen. In V. cholerae, expression of the major virulence factors cholera toxin (CT) and toxin-coregulated pilus (TCP) is regulated by the transcriptional activator ToxT. toxT expression, in turn, is controlled by the transmembrane DNA binding proteins ToxR and TcpP. In the V. cholerae arcA mutant, although ToxR and TcpP were unaffected, Northern blot and reverse transcription-PCR analyses indicated that the expression of toxT was significantly decreased with concomitant reduction in the expression of CT and TCP. CT and TCP expression was completely restored in the V. cholerae arcA mutant strain by expressing a cloned toxT gene in the mutant. These results suggest that ArcA functions as a positive regulator of toxT expression under both aerobic and anaerobic conditions, although as expected, the effect was more pronounced during anaerobic growth. This was reflected in a reduction of virulence of the V. cholerae arcA mutant strain in the infant mouse cholera model.
机译：构建了霍乱弧菌arcA 突变体，并用于检验全球厌氧菌反应调节剂ArcA在该重要人类病原体中毒力因子表达中的作用。在 V中。霍乱毒素，主要毒力因子霍乱毒素（CT）和毒素复合菌毛（TCP）的表达受转录激活因子ToxT的调控。反之， toxT 的表达受跨膜DNA结合蛋白ToxR和TcpP的控制。在 V中。霍乱弧菌arcA 突变体虽然ToxR和TcpP不受影响，但Northern印迹和逆转录-PCR分析表明 toxT 的表达显着降低，同时CT和TCP的表达降低。 CT和TCP表达式在 V中已完全恢复。霍乱弧菌arcA 突变株，通过在该突变株中表达克隆的 toxT 基因来实现。这些结果表明，在有氧和无氧条件下，ArcA都是 toxT 表达的正向调节剂，尽管正如预期的那样，在无氧生长过程中这种作用更为明显。这反映出 V的毒性降低。小鼠霍乱模型中的霍乱弧菌arcA 突变株。

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