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首页> 外文期刊>Infection and immunity >Salmonella enterica Serovar Typhimurium Expressing a Chromosomally Integrated Copy of the Bacillus anthracis Protective Antigen Gene Protects Mice against an Anthrax Spore Challenge
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Salmonella enterica Serovar Typhimurium Expressing a Chromosomally Integrated Copy of the Bacillus anthracis Protective Antigen Gene Protects Mice against an Anthrax Spore Challenge

机译：沙门氏菌血清型鼠伤寒沙门氏菌表达炭疽芽孢杆菌保护性抗原基因的染色体整合拷贝可保护小鼠免受炭疽孢子攻击

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摘要

Protective immunity against infection with Bacillus anthracis is almost entirely based on a response to the protective antigen (PA), the binding moiety for the two other toxin components. We cloned the PA gene into an auxotrophic mutant of Salmonella enterica serovar Typhimurium as a fusion with the signal sequence of the hemolysin (Hly) A gene of Escherichia coli to allow the export of PA via the Hly export system. To stabilize the export cassette, it was also integrated into the chromosome of the live Salmonella carrier. When S. enterica serovar Typhimurium with the chromosomally integrated PA gene was given intravenously to A/J mice, they developed high levels of antibody to PA. These mice were protected against intraperitoneal challenge with 100 or 1,000 50% lethal doses of B. anthracis strain STI. This work contributes to the development of a Salmonella-based orally delivered anthrax vaccine.
机译：对炭疽芽孢杆菌感染的保护性免疫几乎完全基于对保护性抗原（PA）的反应，PA是两种其他毒素成分的结合部分。我们将PA基因克隆到沙门氏菌血清型鼠伤寒营养缺陷型突变体中，将其与溶血素（Hly）的信号序列融合，将大肠杆菌的一个基因允许出口通过Hly导出系统获取PA。为了稳定出口盒，它也被整合到了活的沙门氏菌载体的染色体中。当 S 。将具有染色体整合的PA基因的 enterica 鼠伤寒鼠血清静脉注射给A / J小鼠，它们产生了高水平的PA抗体。用100或1,000的50％致死剂量的 B 预防这些小鼠腹膜内攻击。 炭疽 STI型。这项工作为基于沙门氏菌的口服炭疽疫苗的开发做出了贡献。

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