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首页> 外文期刊>Infection and immunity >Position-Based Scanning for Comparative Genomics and Identification of Genetic Islands in Haemophilus influenzae Type b
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Position-Based Scanning for Comparative Genomics and Identification of Genetic Islands in Haemophilus influenzae Type b

机译：b型流感嗜血杆菌比较基因组学和基于基因岛的基于位置的扫描。

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Bacteria exhibit extensive genetic heterogeneity within species. In many cases, these differences account for virulence properties unique to specific strains. Several such loci have been discovered in the genome of the type b serotype of Haemophilus influenzae, a human pathogen able to cause meningitis, pneumonia, and septicemia. Here we report application of a PCR-based scanning procedure to compare the genome of a virulent type b (Hib) strain with that of the laboratory-passaged Rd KW20 strain for which a complete genome sequence is available. We have identified seven DNA segments or H. influenzae genetic islands (HiGIs) present in the type b genome and absent from the Rd genome. These segments vary in size and content and show signs of horizontal gene transfer in that their percent G+C content differs from that of the rest of the H. influenzae genome, they contain genes similar to those found on phages or other mobile elements, or they are flanked by DNA repeats. Several of these loci represent potential pathogenicity islands, because they contain genes likely to mediate interactions with the host. These newly identified genetic islands provide areas of investigation into both the evolution and pathogenesis of H. influenzae. In addition, the genome scanning approach developed to identify these islands provides a rapid means to compare the genomes of phenotypically diverse bacterial strains once the genome sequence of one representative strain has been determined.
机译：细菌在种内表现出广泛的遗传异质性。在许多情况下，这些差异是特定菌株特有的毒力特性。在流感嗜血杆菌的b型血清型基因组中发现了几个这样的基因座，这是一种能够引起脑膜炎，肺炎和败血病的人类病原体。在这里，我们报告基于PCR的扫描程序的应用程序，以比较强毒的b型（Hib）菌株的基因组与实验室传代的Rd KW20菌株的基因组，该菌株具有完整的基因组序列。我们确定了七个DNA片段或 H。流感病毒遗传岛（HiGI）存在于b型基因组中，而Rd基因组中则没有。这些片段的大小和含量各不相同，并显示出水平基因转移的迹象，因为它们的G + C含量百分比与其余的H含量不同。流感基因组，它们包含的基因类似于在噬菌体或其他移动元件上发现的基因，或者其侧翼是DNA重复序列。这些基因座中的几个代表潜在的致病岛，因为它们包含可能介导与宿主相互作用的基因。这些新近鉴定的遗传岛提供了对 H的进化和发病机理的研究领域。流感。此外，一旦确定了一种代表性菌株的基因组序列，开发用于鉴定这些岛的基因组扫描方法提供了一种比较表型多样的细菌菌株的基因组的快速方法。

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