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首页> 外文期刊>Infection and immunity >Protective Mucosal Th2 Immune Response against Toxoplasma gondii by Murine Mesenteric Lymph Node Dendritic Cells
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Protective Mucosal Th2 Immune Response against Toxoplasma gondii by Murine Mesenteric Lymph Node Dendritic Cells

机译：小鼠肠系膜淋巴结树突状细胞对弓形虫的保护性粘膜Th2免疫反应

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Toxoplasma gondii, an obligate intracellular parasite pathogen which initially invades the intestinal epithelium before disseminating throughout the body, may cause severe sequelae in fetuses and life-threatening neuropathy in immunocompromised patients. Immune protection is usually thought to be performed through a systemic Th1 response; considering the route of parasite entry it is important to study and characterize the local mucosal immune response to T. gondii. Despite considerable effort, Toxoplasma-targeted vaccines have proven to be elusive using conventional strategies. We report the use of mesenteric lymph node dendritic cells (MLNDCs) pulsed ex vivo with T. gondii antigens (TAg) as a novel investigation approach to vaccination against T. gondii-driven pathogenic processes. Using a murine model, we demonstrate in two genetically distinct mouse strains (C57BL/6 and CBA/J) that adoptively transferred TAg-pulsed MLNDCs elicit a mucosal Toxoplasma-specific Th2-biased immune response in vivo and confer strong protection against infection. We also observe that MLNDCs mostly traffic to the intestine where they enhance resistance by reduction in the mortality and in the number of brain cysts. Thus, ex vivo TAg-pulsed MLNDCs represent a powerful tool for the study of protective immunity to T. gondii, delivered through its natural route of entry. These findings might impact the design of vaccine strategies against other invasive microorganisms known to be delivered through digestive tract.
机译：弓形虫是一种专性的细胞内寄生虫病原体，最初会侵入肠道上皮，然后扩散到全身，可能会导致胎儿严重后遗症，并危及免疫功能低下的患者的神经病。通常认为免疫保护是通过全身性Th1反应来完成的。考虑到寄生虫进入的途径，重要的是研究和表征对 T的局部粘膜免疫反应。刚地。尽管付出了巨大的努力，但使用常规策略已证明难以靶向弓形虫的疫苗。我们报道了 T离体脉冲肠系膜淋巴结树突状细胞（MLNDCs）的使用。弓形虫抗原（TAg）作为针对 T的新型疫苗调查方法。刚地驱动的致病过程。使用鼠模型，我们证明了在两个遗传上不同的小鼠品系（C57BL / 6和CBA / J）中，它们过继转移了TAg脉冲的MLNDC，在体内产生了粘膜弓形虫特异性Th2偏向的免疫应答。赋予强大的抗感染保护能力。我们还观察到，MLNDCs大部分流向肠道，在那里它们通过降低死亡率和减少脑囊肿的数量来增强抵抗力。因此，离体TAg脉冲的MLNDCs是研究 T的保护性免疫的有力工具。刚地鱼，通过其自然的进入途径传递。这些发现可能会影响针对已知通过消化道传递的其他侵入性微生物的疫苗策略的设计。

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