Genetic Basis for Biosynthesis of the (α1→4)-Linked N-Acetyl-d-Glucosamine 1-Phosphate Capsule of Neisseria meningitidis Serogroup X

Yih-Ling Tzeng; Corie Noble; David S. Stephens

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Genetic Basis for Biosynthesis of the (α1→4)-Linked N-Acetyl-d-Glucosamine 1-Phosphate Capsule of Neisseria meningitidis Serogroup X

机译：脑膜炎奈瑟氏菌血清群X的（α1→4）-连接的N-乙酰基-d-氨基葡萄糖1-磷酸酯胶囊的生物合成的遗传基础。

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The genetic basis for biosynthesis of the (α1→4)-linked N-acetyl-d-glucosamine 1-phosphate capsule of Neisseria meningitidis serogroup X was defined. The biosynthesis gene cassette was a ～4.2-kb region located between ctrA of the capsule transport operon and galE, which encodes the UDP-glucose-4-epimerase. This location was identical to the locations of the biosynthesis cassettes in other meningococcal serogroups. Three open reading frames unique to meningococcus serogroup X were identified. Deletion-insertion mutation and colony immunoblotting confirmed that these three genes were essential for serogroup X capsule expression, and the genes were designated xcbA, xcbB, and xcbC (serogroup X capsule biosynthesis). Reverse transcriptase PCR indicated that the xcbABC genes form an operon and are cotranscribed divergently from ctrA. XcbA exhibited 52% amino acid similarity to SacB, the putative capsule polymerase of meningococcus serogroup A, suggesting that it plays a role as the serogroup X capsule polymerase. An IS1016 element was found within the intergenic region separating ctrA and xcbA in multiple strains, and this element did not interfere with capsule expression.

机译：定义了脑膜炎奈瑟氏球菌血清群X（α1→4）-连接的 N -乙酰基-d-氨基葡萄糖1-磷酸酯胶囊生物合成的遗传基础。生物合成基因盒位于胶囊转运操纵子 ctrA 和 galE 之间约4.2kb的区域，编码UDP-葡萄糖-4-表观异构酶。该位置与其他脑膜炎球菌血清群中生物合成盒的位置相同。确定了三个脑膜炎双球菌血清群X特有的开放阅读框。缺失插入突变和菌落免疫印迹证实这三个基因是血清群X胶囊表达所必需的，这些基因分别命名为 xcbA ， xcbB 和 xcbC （血清群X胶囊的生物合成）。逆转录PCR显示 xcbABC 基因形成操纵子，并从 ctrA 共转录。 XcbA与SacB（脑膜炎球菌血清群A的推定的胶囊聚合酶）具有52％的氨基酸相似性，表明它作为X血清群胶囊聚合酶起作用。在多个菌株中分离 ctrA 和 xcbA 的基因间区域内发现了一个IS 1016 元件，该元件不干扰胶囊的表达。 展开▼

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《Infection and immunity》 |2003年第12期|共9页

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Yih-Ling Tzeng; Corie Noble; David S. Stephens;
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中图分类医学免疫学;

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专利

1. Identification of a genetic locus involved in the biosynthesis of N-acetyl-D-mannosamine, a precursor of the (alpha 2-->8)-linked polysialic acid capsule of serogroup B Neisseria meningitidis. [J] . J S Swartley, D S Stephens Journal of bacteriology . 1994,第5期

机译：鉴定涉及N-乙酰基-D-甘露糖胺生物合成的遗传基因座，N-乙酰基-D-甘露糖胺是脑膜炎奈瑟氏球菌血清群（α2-→8）连接的多唾液酸胶囊的前体。

2. Identification of a genetic locus involved in the biosynthesis of N-acetyl-D-mannosamine, a precursor of the (alpha 2-->8)-linked polysialic acid capsule of serogroup B Neisseria meningitidis. [J] . J S Swartley, D S Stephens Journal of bacteriology . 1994,第5期

机译：鉴定涉及N-乙酰基-D-甘露糖胺生物合成的遗传基因座，N-乙酰基-D-甘露糖胺是脑膜炎奈瑟氏球菌血清群（α2-→8）连接的多唾液酸胶囊的前体。

3. Characterization of the Gene Cassette Required for Biosynthesis of the (α1→6)-LinkedN-Acetyl-d-Mannosamine-1-Phosphate Capsule of Serogroup A Neisseria meningitidis [J] . John S. Swartley, Li-Jun Liu, Yoon K. Miller, Journal of bacteriology . 1998,第6期

机译：脑膜炎奈瑟氏球菌（α1→6）-连接的N-乙酰基-d-甘露糖胺-1-磷酸胶囊生物合成所需的基因盒的表征

4. Fatal meningococcal meningitidis in a baby caused by serogroup C Neisseria meningitidis belonging to ST-4821 complex [C] . Hui Han, Haishan Li, Yu Yang, International Conference on Human Health and Medical Engineering. . 2014

机译：致命的脑膜炎球菌脑膜脑膜脑膜脑膜脑膜炎症患者由Serogroup C Neisseria Meningitidis属于ST-4821复合物

5. Biochemical Assays of the Neisseria meningitidis Serogroup W Capsule Polymerase [D] . Sharyan, Abeer. 2018

机译：Neisseria Meningitidis血清群的生化测定胶囊聚合酶

6. Genetic Basis for Biosynthesis of the (α1→4)-Linked N-Acetyl-d-Glucosamine 1-Phosphate Capsule of Neisseria meningitidis Serogroup X [O] . Yih-Ling Tzeng, Corie Noble, David S. Stephens 2003

机译：生物合成的遗传基础（α1→4）-链接 N-乙酰基-d-氨基葡萄糖1-磷酸酯胶囊脑膜炎奈瑟氏球菌血清群 X

7. Genetic Basis for Biosynthesis of the (α1→4)-Linked N-Acetyl-d-Glucosamine 1-Phosphate Capsule of Neisseria meningitidis Serogroup X [O] . Tzeng, Yih-Ling, Noble, Corie, Stephens, David S. 2003

机译：生物合成的遗传基础（α1→4）-链接 N-乙酰基-d-氨基葡萄糖1-磷酸酯胶囊脑膜炎奈瑟氏球菌血清群 X

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机译：多克隆抗体，诊断试剂，脑膜炎奈瑟菌血清群x体外检测方法，脑膜炎奈瑟氏菌血清群x感染体外诊断方法，试剂盒和诊断测试，诊断试剂盒的使用和抗体生产方法

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Genetic Basis for Biosynthesis of the (α1→4)-Linked N-Acetyl-d-Glucosamine 1-Phosphate Capsule of Neisseria meningitidis Serogroup X

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