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首页> 外文期刊>Infection and immunity >Immunosuppressive factor from Actinobacillus actinomycetemcomitans down regulates cytokine production.
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Immunosuppressive factor from Actinobacillus actinomycetemcomitans down regulates cytokine production.

机译：来自放线放线杆菌的免疫抑制因子下调细胞因子的产生。

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A cytoplasmic soluble fraction of Actinobacillus actinomycetemcomitans Y4 was isolated and characterized as suppressing mitogen-stimulated proliferation of and cytokine production by C3H/HeN mouse splenic T cells. This factor, designated suppressive factor 1 (SF1), was isolated from the supernatant of sonicated whole bacteria and purified by Q-Sepharose Fast Flow column chromatography, DEAE-Sepharose Fast Flow column chromatography, hydroxyapatite high-pressure liquid chromatography (HPLC), and Protein Pack 300 & 125 gel filtration HPLC. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that the purified SF1 migrated as a single band corresponding to a molecular mass of 14 kDa. This molecule was protease labile, heat resistant, and noncytotoxic. N'-terminal sequence analysis revealed no homology with any known peptides of periodontopathic bacteria or with any host-derived growth factors. Purified SF1 suppressed the proliferation of mouse splenic T cells which had been stimulated with concanavalin A, as well as suppressing the production of interleukin-2 (IL-2), gamma interferon, IL-4, and IL-5 from CD4+ T cells as 0.1 microgram/ml or more. These data suggest that SF1 produced by the periodontal pathogen A. actinomycetemcomitans functions as a virulence factor by down regulating T-cell proliferation and cytokine production at local defense sites.
机译：分离了放线放线放线杆菌Y4的细胞质可溶级分，并表征为抑制丝裂原刺激的C3H / HeN小鼠脾T细胞的增殖和细胞因子的产生。从超声处理的完整细菌的上清液中分离出该因子，称为抑制因子1（SF1），并通过Q-Sepharose Fast Flow柱色谱，DEAE-Sepharose Fast Flow柱色谱，羟基磷灰石高压液相色谱（HPLC）和Protein Pack 300和125凝胶过滤HPLC。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析表明，纯化的SF1以单条带迁移，对应于14 kDa的分子量。该分子对蛋白酶不稳定，耐热且无细胞毒性。 N'末端序列分析显示与牙周病细菌的任何已知肽或任何宿主来源的生长因子均无同源性。纯化的SF1抑制了伴刀豆球蛋白A刺激的小鼠脾T细胞的增殖，并抑制了CD4 + T细胞产生白介素2（IL-2），γ干扰素，IL-4和IL-5的产生。 0.1微克/毫升或更高。这些数据表明，由牙周病原体放线杆菌产生的SF1通过下调局部防御位点的T细胞增殖和细胞因子产生而成为一种毒力因子。

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