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首页> 外文期刊>Infection and immunity >Actinobacillus actinomycetemcomitans serotype b-specific polysaccharide antigen stimulates production of chemotactic factors and inflammatory cytokines by human monocytes.
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Actinobacillus actinomycetemcomitans serotype b-specific polysaccharide antigen stimulates production of chemotactic factors and inflammatory cytokines by human monocytes.

机译：放线杆菌放线杆菌血清型b特异性多糖抗原刺激人单核细胞产生趋化因子和炎性细胞因子。

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摘要

Serotype b-specific polysaccharide antigen (SPA) was extracted from whole cells of Actinobacillus actinomycetemcomitans Y4 by autoclaving and purified by chromatography on DEAE-Sephadex A-25 and Sephacryl S-300. SPA induced the release of monocyte and leukocyte chemotactic factors by human monocytes. Polymyxin B had almost no effect on the release of monocyte chemotactic factor, but a monoclonal antibody against SPA markedly inhibited it. Human monocytes stimulated with SPA exhibited the increased mRNA expression of monocyte chemoattractant protein 1 (MCP-1) and a neutrophil chemotactic factor, interleukin-8 (IL-8). On the other hand, SPA induced the release of IL-1, IL-6, and tumor necrosis factor (TNF) and enhanced the expression of IL-1alpha, IL-1beta, IL-6, and TNF alpha (TNF-alpha) mRNAs. Human monocytes expressed MCP-1 and IL-8 mRNAs when stimulated by human recombinant IL-1alpha, I1-1beta, IL-6, and TNF-alpha, suggesting that these inflammatory cytokines induced by SPA might participate in the production of chemotactic factors in human monocytes.
机译：通过高压灭菌从放线放线放线杆菌Y4的全细胞中提取血清型b特异性多糖抗原（SPA），并在DEAE-Sephadex A-25和Sephacryl S-300上进行色谱纯化。 SPA诱导人单核细胞释放单核细胞和白细胞趋化因子。多粘菌素B几乎对单核细胞趋化因子的释放没有影响，但是针对SPA的单克隆抗体明显抑制了它。用SPA刺激的人单核细胞显示单核细胞趋化蛋白1（MCP-1）和嗜中性粒细胞趋化因子白介素8（IL-8）的mRNA表达增加。另一方面，SPA诱导IL-1，IL-6和肿瘤坏死因子（TNF）的释放，并增强IL-1alpha，IL-1beta，IL-6和TNF alpha（TNF-alpha）的表达。 mRNA。当人重组IL-1alpha，I1-1beta，IL-6和TNF-alpha刺激时，人单核细胞表达MCP-1和IL-8 mRNA，表明SPA诱导的这些炎性细胞因子可能参与了趋化因子的产生。人单核细胞。

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