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Identification of the Helicobacter pylori VacA Toxin Domain Active in the Cell Cytosol

机译：在细胞液中有活性的幽门螺杆菌VacA毒素域的鉴定

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Cells exposed to Helicobacter pylori toxin VacA develop large vacuoles which originate from massive swelling of membranous compartments at late stages of the endocytic pathway. When expressed in the cytosol, VacA induces vacuolization as it does when added from outside. This and other evidence indicate that VacA is a toxin capable of entering the cell cytosol, where it displays its activity. In this study, we have used cytosolic expression to identify the portion of the toxin molecule responsible for the vacuolating activity. VacA mutants with deletions at the C and N termini were generated, and their activity was analyzed upon expression in HeLa cells. We found that the vacuolating activity of VacA resides in the amino-terminal region, the whole of which is required for its intracellular activity.
机译：暴露于幽门螺杆菌毒素VacA的细胞会形成较大的液泡，其起源于内吞途径后期膜腔室的大量肿胀。当在细胞质中表达时，VacA诱导空泡化，就像从外部添加时一样。该证据和其他证据表明，VacA是一种能够进入细胞溶质并在其中显示其活性的毒素。在这项研究中，我们已经使用了胞质表达来确定毒素分子负责空泡活性的部分。产生在C和N末端具有缺失的VacA突变体，并在HeLa细胞中表达后对其活性进行分析。我们发现，VacA的空泡活性位于氨基末端区域，这是其细胞内活性所必需的。

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