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首页> 外文期刊>Infection and immunity >Molecular Cloning and Characterization of the Genes Coding for the Highly Immunogenic Cluster of 90-Kilodalton Envelope Proteins from the Chlamydia psittaci Subtype That Causes Abortion in Sheep
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Molecular Cloning and Characterization of the Genes Coding for the Highly Immunogenic Cluster of 90-Kilodalton Envelope Proteins from the Chlamydia psittaci Subtype That Causes Abortion in Sheep

机译：导致绵羊流产的衣原体亚型衣原体的90-Kilodalton信封蛋白的高度免疫原性簇的基因编码和分子克隆。

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Proteins present in the outer membrane of chlamydiae that are involved in mucosal epithelial cell infection must clearly be identified and characterized if we are to understand and modify the pathogenic mechanisms utilized by these organisms. We have identified and isolated a family of four genes encoding putative outer membrane proteins (POMPs), a group of proteins of approximately 90 kDa present in the outer membrane of the subtype of Chlamydia psittacithat causes ovine enzootic abortion (strain S26/3). These proteins, although minor components, are major immunogens, as shown by the immunoblotting of chlamydial outer membrane complexes with postabortion sheep sera, and are therefore potential diagnostic and/or protective antigen candidates. Immunoblotting of the expressed amino- and carboxy-terminal halves of one of the POMPs with postabortion sheep sera showed that the major humoral immune response appeared to be directed solely against the amino-terminal half. This result, in combination with the positive immunofluorescence staining of S26/3-infected cells using POMP-specific (specific to the amino-terminal half of the proteins) monoclonal antibodies, suggests the probable surface localization of the POMPs and, more specifically, the surface exposure of the amino-terminal half of these proteins. The four pomp genes are highly homologous, sharing 82 to 100% similarity with each other (two of the genes are identical). Genes with strong and weak homologies were also detected in C. psittaci avian and feline pneumonitis strains, respectively. Nopomp homologs were found in strains of C. trachomatis and C. pneumoniae, but this does not preclude their existence. The absence of homology with various subtypes of C. pecorum, which complicate the diagnosis of the ovine abortion subtype, indicates the possible suitability of the these 90-kDa proteins as serodiagnostic antigens.
机译：如果我们要了解和修改这些生物利用的致病机制，则必须清楚地鉴定和表征衣原体外膜中与粘膜上皮细胞感染有关的蛋白质。我们已经鉴定并分离出了一个编码推定外膜蛋白（POMPs）的四个基因家族，这四个基因存在于导致羊胎动物流产的 psilamed psittaci 亚型的外膜中约90 kDa的蛋白。 （应变S26 / 3）。这些蛋白尽管是次要成分，但是却是主要的免疫原，如衣原体外膜复合物与堕胎后绵羊血清的免疫印迹法所示，因此是潜在的诊断和/或保护性抗原候选物。用流产后绵羊血清对其中一个POMP的表达的氨基末端和羧基末端一半进行免疫印迹分析，结果表明，主要的体液免疫应答似乎仅针对氨基末端一半。该结果与使用POMP特异性（对蛋白质的氨基末端一半特异性）单克隆抗体对S26 / 3感染的细胞进行的阳性免疫荧光染色相结合，表明POMP可能存在表面定位，更具体地讲，这些蛋白质氨基末端一半的表面暴露。四个 pomp 基因高度同源，彼此共有82至100％的相似性（两个基因相同）。在 C中也检测到具有强同源性和弱同源性的基因。 psittaci 禽和猫肺炎菌株。在 C菌株中未发现 pomp 同源物。沙眼和 C。肺炎，但这并不排除它们的存在。与 C的各种亚型没有同源性。 pecorum 使绵羊流产亚型的诊断复杂化，这表明这些90 kDa蛋白可能适合作为血清诊断抗原。

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