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首页> 外文期刊>Infection and immunity >Legionella pneumophila Utilizes the Same Genes To Multiply within Acanthamoeba castellanii and Human Macrophages
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Legionella pneumophila Utilizes the Same Genes To Multiply within Acanthamoeba castellanii and Human Macrophages

机译：嗜肺军团菌利用相同的基因在棘阿米棘和人类巨噬细胞内繁殖

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In previous reports we described a 22-kb Legionella pneumophila chromosomal locus containing 18 genes. Thirteen of these genes (icmT, -R, -Q, -P, -O, -M, -L, -K, -E, -C, -D, -J, and -B) were found to be completely required for intracellular growth and killing of human macrophages. Three genes (icmS, -G, and -F) were found to be partially required, and two genes (lphA andtphA) were found to be dispensable for intracellular growth and killing of human macrophages. Here, we analyzed the requirement of these genes for intracellular growth in the protozoan hostAcanthamoeba castellanii, a well-established important environmental host of L. pneumophila. We found that all the genes that are completely required for intracellular growth in human macrophages are also completely required for intracellular growth in A. castellanii. However, the genes that are partially required for intracellular growth in human macrophages are completely required for intracellular growth in A. castellanii. In addition, the lphA gene, which was shown to be dispensable for intracellular growth in human macrophages, is partially required for intracellular growth in A. castellanii. Our results indicate that L. pneumophila utilizes the same genes to grow intracellularly in both human macrophages and amoebae.
机译：在以前的报告中，我们描述了一个包含18个基因的22 kb 嗜肺军团菌染色体位点。这些基因中的13个（ icmT ，- R ，- Q ，- P ，- O < / em>，- M ，- L ，- K ，- E ，- C < / em>，- D ，- J 和- B ）完全是细胞内生长和杀死人类巨噬细胞所必需的。发现部分需要三个基因（ icmS ，- G 和- F ），两个基因（ lphA 和 tphA ）对于细胞内生长和杀死人类巨噬细胞是必不可少的。在这里，我们分析了这些基因对原生动物宿主 Acanthamoeba castellanii （一种成熟的 L重要宿主）细胞内生长的需求。肺炎。我们发现，人类巨噬细胞中细胞内生长完全需要的所有基因也完全被 A细胞内生长所需。 Castellanii 。然而，人类巨噬细胞中细胞内生长所需的部分基因完全是 A中细胞内生长所需的。 Castellanii 。另外，显示 lphA 基因对于人类巨噬细胞的细胞内生长是可有可无的，而部分 A的细胞内生长则是必需的。 Castellanii 。我们的结果表明 L。肺炎利用相同的基因在人类巨噬细胞和变形虫中细胞内生长。

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