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首页> 外文期刊>Infection and immunity >Infection of Human Endothelial Cells with Bartonella bacilliformis Is Dependent on Rho and Results in Activation of Rho
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Infection of Human Endothelial Cells with Bartonella bacilliformis Is Dependent on Rho and Results in Activation of Rho

机译：杆状芽孢杆菌感染人内皮细胞取决于Rho及其激活Rho的结果

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Bartonella bacilliformis was continuously internalized into human endothelial cells beginning shortly after addition of the bacteria and continuing for at least 24 h after infection in vitro, with a major increase in uptake occurring between 16 and 24 h. Preincubation of endothelial cells with C3 exoenzyme, which inactivated intracellular Rho-GTPase, blocked internalization of the bacteria. Addition of C3 exoenzyme at any time after addition of the bacteria blocked further internalization of bacteria, including the major uptake of bacteria internalized at 16 to 24 h. Rho, a key signaling protein in pathways involving actin organization, was directly shown to be activated in endothelial cells undergoing infection with B. bacilliformis, with maximal activation and translocation to the plasma membrane at 12 to 16 h. At late times of infection, most of the bacteria were found in a perinuclear location. Staining of the Golgi complex with specific markers, anti-human Golgin-97, anti-KDEL receptor, and BODIPY-TR ceramide, showed colocalization of bacteria in the Golgi complex region. Disruption of the Golgi complex with brefeldin A scattered the bacteria from this perinuclear location and resulted in inhibition of internalization of the bacteria in endothelial cells.
机译：在细菌添加后不久，细菌细菌巴尔通体不断被内化到人内皮细胞中，并在体外感染后持续至少24小时，摄取的主要增加发生在16至24小时之间。用灭活细胞内Rho-GTPase的C3外切酶对内皮细胞进行预培养，可阻止细菌的内在化。在添加细菌后的任何时间添加C3外酶均会阻止细菌的进一步内在化，包括在16至24小时内摄取的大量内在细菌。 Rho是涉及肌动蛋白组织的途径中的关键信号转导蛋白，已直接显示在受到 B感染的内皮细胞中被激活。芽孢杆菌，在12至16小时内具有最大的活化和转移到质膜的能力。在感染后期，大多数细菌位于核周位置。用特定标记，抗人Golgin-97，抗KDEL受体和BODIPY-TR神经酰胺对高尔基复合体染色表明，细菌在高尔基复合体区域共定位。高尔基复合体与布雷菲德菌素A的破坏使细菌从该核周位置散开，并抑制了细菌在内皮细胞中的内在化。

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