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首页> 外文期刊>Infection and immunity >Inducible Expression of Enterococcus faecalis Aggregation Substance Surface Protein Facilitates Bacterial Internalization by Cultured Enterocytes
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Inducible Expression of Enterococcus faecalis Aggregation Substance Surface Protein Facilitates Bacterial Internalization by Cultured Enterocytes

机译：粪肠球菌聚集物质表面蛋白的诱导表达通过培养的肠上皮细胞促进细菌内在化

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摘要

Aggregation substance (AS) is an Enterococcus faecalissurface protein that may contribute to virulence. Using a recently described system for controlled expression of AS in E. faecalis and the heterologous host Lactococcus lactis, experiments were designed to assess the effect of AS on bacterial internalization by HT-29 and Caco-2 enterocytes. AS expression was associated with increased internalization of E. faecalis by HT-29 enterocytes and of L. lactis by HT-29 and Caco-2 enterocytes. Compared to enterocytes cultivated under standard conditions, either cultivation in hypoxia or 1-h pretreatment of enterocytes with calcium-free medium resulted in increased internalization of both E. faecalis and L. lactis (with and without AS expression). Also, AS expression augmented these increases when E. faecalis was incubated with pretreated HT-29 enterocytes and when L. lactis was incubated with pretreated Caco-2 and HT-29 enterocytes. These data indicated that AS might facilitate E. faecalisinternalization by cultured enterocytes.
机译：聚集物质（AS）是粪肠球菌（emococcus faecalis）的表面蛋白，可能有助于致病性。使用最近描述的系统在 E中控制AS的表达。粪便和异源宿主乳酸乳球菌，设计实验以评估AS对HT-29和Caco-2肠上皮细胞细菌内在化的影响。 AS表达与 E的增加的内在化相关。粪肠杆菌通过HT-29肠细胞和 L。 HT-29和Caco-2肠上皮细胞分泌乳酸菌。与标准条件下培养的肠上皮细胞相比，在缺氧条件下培养或用无钙培养基对肠上皮细胞进行1小时预处理会导致两种 E的内在化程度提高。粪便和 L。乳酸（带有和不带有AS表达）。同样，当 E时，AS表达增加了这些增加。粪便与预处理的HT-29肠上皮细胞以及 L孵育。乳酸与预处理的Caco-2和HT-29肠上皮细胞一起孵育。这些数据表明，AS可能有助于 E。粪便通过培养的肠细胞内化。

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