...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Minor Nucleotide Substitutions in the omp31 Gene ofBrucella ovis Result in Antigenic Differences in the Major Outer Membrane Protein That It Encodes Compared to Those of the OtherBrucella Species
【24h】

Minor Nucleotide Substitutions in the omp31 Gene ofBrucella ovis Result in Antigenic Differences in the Major Outer Membrane Protein That It Encodes Compared to Those of the OtherBrucella Species

机译：绵羊小球藻omp31基因中的小核苷酸取代导致编码的主要外膜蛋白与其他小球藻物种相比在抗原上的差异

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The gene coding for the major outer membrane protein Omp31 was sequenced in five Brucella species and their biovars. Although the omp31 genes appeared to be highly conserved in the genus Brucella, nine nucleotide substitutions were detected in the gene of Brucella ovis compared to that ofBrucella melitensis. As shown by differential binding properties of monoclonal antibodies (MAbs) to the twoBrucella species, these nucleotide substitutions result in different antigenic properties of Omp31. The antigenic differences were also evidenced when sera from B. ovis-infected rams were tested by Western blotting with the recombinant B. melitensis or B. ovis Omp31 proteins. Twelve available sera reacted with recombinant B. ovis Omp31, but only four of them reacted with recombinant B. melitensisOmp31. These results validate previous evidence for the potential of Omp31 as a diagnostic antigen for B. ovis infection in rams and demonstrate that B. ovis Omp31, instead of B. melitensis Omp31, should be used to evaluate this point. The antigenic differences between the B. melitensis andB. ovis Omp31 proteins should also be taken into account when Omp31 is evaluated as a candidate for the development of subcellular vaccines against B. ovis infection. No reactivity against recombinant B. melitensis Omp31 was detected, by Western blotting, with sera from B. melitensis-infected sheep. Accordingly, Omp31 does not seem to be a good diagnostic antigen for B. melitensis infections in sheep. Two immunodominant regions were identified on the B. ovis Omp31 protein by using recombinant DNA techniques and specific MAbs. Sera from B. ovis-infected rams that reacted with the recombinant protein were tested by Western blotting against one of these immunodominant regions shown to be exposed at the bacterial surface. Only 4 of the 12 sera reacted, but with strong intensity.
机译：在五个 Brucella 物种及其生物变量中对主要外膜蛋白Omp31的编码基因进行了测序。尽管 Brucella 属中的 omp31 基因高度保守，但是与相比，在 Brucella ovis 基因中检测到了九个核苷酸取代。 布鲁氏菌。如单克隆抗体（MAb）对两种 Brucella 物种的结合特性不同所示，这些核苷酸取代导致Omp31的抗原特性不同。当来自 B的血清时，也证明了抗原差异。通过重组 B的Western印迹检测被ovis 感染的公羊。 lit 或 B。 ovis Omp31蛋白。十二个可用血清与重组B发生反应。 ovis Omp31，但其中只有四个与重组 B反应。蜜蜂 Omp31。这些结果验证了以前的证据表明Omp31可能作为B的诊断抗原。 ovis 感染了公羊，并证明了 B。 ovis Omp31，而不是 B。应该使用melitensis Omp31来评估这一点。 B之间的抗原差异。 lit 和 B。当Omp31被评估为开发针对 B的亚细胞疫苗的候选者时，也应考虑ovis Omp31蛋白。 ovis 感染。对重组 B无反应性。用蛋白质印迹法检测了 B血清中的melitensis Omp31。感染了羊肠病毒的绵羊。因此，Omp31似乎不是B的良好诊断抗原。绵羊感染了melitensis 。在 B上鉴定出两个免疫优势区域。 ovis Omp31蛋白，采用重组DNA技术和特异性单克隆抗体。 B的血清。通过Western印迹针对显示出暴露于细菌表面的这些免疫优势区域之一，测试了与重组蛋白反应的经ovis感染的公羊。 12个血清中只有4个发生了反应，但强度很高。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号